Study on the value of second-generation sequencing technology in the clinical diagnosis of osteoarticular brucellosis.

Publisher: Wiley Country of Publication: United States NLM ID: 8404726 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1554-527X (Electronic) Linking ISSN: 07360266 NLM ISO Abbreviation: J Orthop Res Subsets: MEDLINE

Publication: 2006- : Hoboken, NJ : Wiley
Original Publication: New York, N.Y. : Raven Press, [c1983-

Brucellosis*/diagnosis ; High-Throughput Nucleotide Sequencing*; Humans ; Male ; Middle Aged ; Female ; Retrospective Studies ; Adult ; Aged ; Agglutination Tests ; Sensitivity and Specificity

To assess the value of next-generation sequencing (NGS) technology in the diagnosis of osteoarticular brucellosis pathogenesis. Fifty eight patients admitted to the Department of Orthopaedics, Hebei Provincial Chest Hospital from January 2021 to January 2023 were retrospectively analyzed, and the patients were classified into 48 cases in the osteoarticular brucellosis group and 10 cases in the nonosteoarticular brucellosis group according to the final clinical diagnosis. All patients underwent serum agglutination test (SAT), CT-guided puncture or surgical sampling of lesions for bacteriological culture and NGS after admission. The diagnostic efficacy of these three methods for osteoarticular brucellosis was compared using the final clinical diagnosis as the reference standard. Among the 58 patients with suspected osteoarticular brucellosis, 40 cases (68.97%) were positive by NGS, 33 cases (56.89%) by SAT and 10 cases (17.24%) by culture, and the differences were statistically significant (p < 0.05). Using the final clinical diagnosis as a criterion, the sensitivity of NGS, SAT, and culture for the detection of osteoarticular brucellosis was 83.33%, 62.50%, and 20.83%, respectively, the specificity was 100.00%, 70.00%, and 100.00%, the diagnostic accuracy was 86.20%, 63.79%, and 34.49%, and the κ values were 0.799, 0.590, and 0.504, respectively. NGS has a high pathogen detection rate and sensitivity in the pathogenetic diagnosis of patients with osteoarticular brucellosis and can provide clinical guidance for the diagnosis and management of patients with osteoarticular brucellosis.
(© 2024 The Authors. Journal of Orthopaedic Research® published by Wiley Periodicals LLC on behalf of Orthopaedic Research Society.)

Ye H, Gao R, Zhang J, et al. Comparative study of metagenomics next generation sequencing of cerebrospinal fluid in the diagnosis test of the pathogen of neurobrucellosis. Chin J Neurol. 2021;54(11):1128‐1133. doi:10.3760/cma.j.cn113694-20210320-00204.
Miller S, Chiu C. The role of metagenomics and next‐generation sequencing in infectious disease diagnosis. Clin Chem. 2021;68(1):115‐124. doi:10.1093/clinchem/hvab173.
Zeng J, Zhao H, Yin D, Zheng Z, Lv Y, Qin C. A case of Borrelia burgdorferi spondylitis diagnosed by using mNGS method. Chin J Bone Joint Injury. 2019;34(1):103‐104. doi:10.7531/j.issn.1672-9935.2019.01.037.
Ministry of Health of the People's Republic of China. Brucellosis guidelines (trial implementation). Infect Dis Info. 2012;25(6):323‐324. doi:10.3969/j.issn.1007-8134.201.
Editorial Board of “Chinese Journal of Infectious Diseases”. Expert consensus on the diagnosis and treatment of brucellosis. Chin J Infect Dis. 2017;35(12):705‐710. doi:10.3760/cma.j.issn.1000-6680.2017.12.001.
Gao Y, Li S, Du X. Diagnostic value of tube agglutination test and tiger red plate agglutination test for brucellosis. Clin Res Pract. 2021;6(5):114‐115. doi:10.19347/j.cnki.2096-1413.202105041.
Wang ZQ, Wang JQ. Diagnosis and differential diagnosis of Brucella spondylitis. Chin J Control Endem Dis. 2010;25(5):339‐340.
Zhai JB, Lv CL, Chen YS, Gao W, Zhao LH. Method for Detecting Brucella Infection and Application Thereof. United States Patent and Trademark Office, US11505834B2; 2022:11‐22.
Esmaeilnejad‐Ganji SM, Esmaeilnejad‐Ganji SMR. Osteoarticular manifestations of human brucellosis: a review. World J Orthop. 2019;10(2):54‐62. doi:10.5312/wjo.v10.i2.54.
Wang J, Zhang Q. Progress in the diagnosis and treatment of Brucella spondylitis. Orthop J China. 2021;29(14):1304‐1307. doi:10.3977/j.issn.1005-8478.2021.14.13.
Jin K, Wang X, Guan H, et al. Application of next‐generation sequencing in the Brucella infection in the central nervous system. Chin J Clin Infect Dis. 2020;13(3):195‐198. doi:10.3760/cma.j.issn.1674-2397.2020.03.007.
You J, Li H. Application and progress of second‐generation sequencing in the diagnosis of neuroborreliosis. Xinjiang Med J. 2020;50(01):85‐88.
Elbehiry A, Aldubaib M, Marzouk E, et al. The development of diagnostic and vaccine strategies for early detection and control of human brucellosis, particularly in endemic areas. Vaccines. 2023;11(3):654. doi:10.3390/vaccines11030654.
Yagupsky P, Morata P, Colmenero JD. Laboratory diagnosis of human brucellosis. Clin Microbiol Rev. 2019;33(1):e00073. doi:10.1128/CMR.00073-19.
Zhang L, Sun S, Li W, Wang X. Diagnosis of Brucella infection using metagenomic next generation sequencing after total knee arthroplasty: a case report. Chin J Geriatr. 2020;39(11):1340‐1341. doi:10.3760/cma.j.issn.0254-9026.2020.11.022.
Dadar M, Alamian S, Brangsch H, et al. Determination of virulence‐associated genes and antimicrobial resistance profiles in Brucella isolates recovered from humans and animals in Iran using NGS technology. Pathogens. 2023;12(1):82. doi:10.3390/pathogens12010082.
Fan S, Ren H, Wei Y, et al. Next‐generation sequencing of the cerebrospinal fluid in the diagnosis of neurobrucellosis. Int J Infect Dis. 2018;67:20‐24. doi:10.1016/j.ijid.2017.11.028.
Zhao M, Tang K, Liu F, et al. Metagenomic next‐generation sequencing improves diagnosis of osteoarticular infections from abscess specimens: a multicenter retrospective study. Front Microbiol. 2020;11:2034. doi:10.3389/fmicb.2020.02034.
Zhou X, Wu H, Ruan Q, et al. Clinical evaluation of diagnosis efficacy of active mycobacterium tuberculosis complex infection via metagenomic next‐generation sequencing of direct clinical samples. Front Cell Infect Microbiol. 2019;9:351. doi:10.3389/fcimb.2019.00351.
Geng S, Mei Q, Zhu C, et al. Metagenomic next‐generation sequencing technology for detection of pathogens in blood of critically ill patients. Int J Infect Dis. 2021;103:81‐87. doi:10.1016/j.ijid.2020.11.166.
Moriarty TF, Hickok NJ, Saeed K, Schaer TP, Chen AF, Schwarz EM. The 2023 Orthopaedic Research Society International Consensus Meeting on musculoskeletal infection. J Orthop Res. 2024;42(3):497‐499. doi:10.1002/jor.25714.
Schwarz EM, Archer NK, Atkins GJ, et al. The 2023 Orthopaedic Research Society's International Consensus Meeting on musculoskeletal infection: summary from the host immunity section. J Orthop Res. 2024;42(3):518‐530. doi:10.1002/jor.25758.
Hickok NJ, Li B, Oral E, et al. The 2023 Orthopedic Research Society's international consensus meeting on musculoskeletal infection: summary from the in vitro section. J Orthop Res. 2024;42(3):512‐517. doi:10.1002/jor.25774.
Jennings JA, Arts JJ, Abuhussein E, et al. 2023 International Consensus Meeting on musculoskeletal infection: summary from the treatment workgroup and consensus on treatment in preclinical models. J Orthop Res. 2024;42(3):500‐511. doi:10.1002/jor.25765.

20191000 2019 Medical Science Research Project in Hebei Province

Keywords: brucellosis; diagnostic techniques; genomic II sequencing; osteoarthritis

Date Created: 20240509 Date Completed: 20241017 Latest Revision: 20241017

20241018

10.1002/jor.25867

38722074