[Transdermal patches containing Cassia seed extract applied at the navel for slow transit constipation in rats: therapeutic effect and analysis of the spectrum-effect relationship].

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  • Author(s): Huang L;Huang L; Cheng S; Cheng S; Liu Z; Liu Z; Zou C; Zou C; Yan H; Yan H
  • Source:
    Nan fang yi ke da xue xue bao = Journal of Southern Medical University [Nan Fang Yi Ke Da Xue Xue Bao] 2024 Apr 20; Vol. 44 (4), pp. 720-726.
  • Publication Type:
    English Abstract; Journal Article
  • Language:
    Chinese
  • Additional Information
    • Source:
      Publisher: Nanfang yi ke da xue xue bao bian ji bu Country of Publication: China NLM ID: 101266132 Publication Model: Print Cited Medium: Print ISSN: 1673-4254 (Print) Linking ISSN: 16734254 NLM ISO Abbreviation: Nan Fang Yi Ke Da Xue Xue Bao Subsets: MEDLINE
    • Publication Information:
      Original Publication: Guangzhou : Nanfang yi ke da xue xue bao bian ji bu, 2005-
    • Subject Terms:
      Cassia*/chemistry ; Constipation*/drug therapy ; Seeds*/chemistry ; Transdermal Patch* ; Plant Extracts*; Animals ; Rats ; Rats, Sprague-Dawley ; Colon/drug effects ; Acupuncture Points ; Nitric Oxide/metabolism ; Disease Models, Animal ; Male ; Drugs, Chinese Herbal/therapeutic use
    • Abstract:
      Objective: To explore the therapeutic effect of transdermal patches containing Cassia seed extract applied at the navel on slow transit constipation (STC) in rats and explore the spectrum-effect relationship of the patches.
      Method: In a STC rat model established by gavage of compound diphenoxylate suspension for 14 days, the transdermal patches containing low, medium and high doses of Cassia seed extract (41.75, 125.25, and 375.75 mg/kg, respectively) were applied at the Shenque acupoint on the abdomen for 14 days after modeling, with constipation patches (13.33 mg/kg) as the positive control. After the treatment, fecal water content and intestinal propulsion rate of the rats were calculated, the pathological changes in the colon were observed with HE staining. Serum NO and NOS levels and the total protein content and NO, NOS and AChE expressions in the colon tissue were determined. HPLC fingerprints of the transdermal patches were established, and the spectrum-effect relationship between the common peaks of the patches and its therapeutic effect were analyzed.
      Results: Treatment with the transdermal patches containing Cassia seed extract significantly increased fecal water content and intestinal propulsion rate of the rat models, where no pathological changes in the colon tissue were detected. The treatment also suppressed the elevations of serum and colonic NO and NOS levels and reduction of AChE in STC rats. Twenty-eight common peaks were confirmed in the HPLC fingerprints of 6 batches of Cassia seed extract-containing patches. Analysis of the spectrum-effect relationship showed that autrantio-obtusin had the greatest contribution to the therapeutic effect of the patches in STC rats.
      Conclusion: The Cassia seed extract-containing patches alleviates STC in rats via synergistic actions of multiple active ingredients in the extract, where autrantio-obtusin, rhein, chrysoobtusin, obtusin, obtusifolin, emodin, chrysophanol, and physcion are identified as the main active ingredients.
    • References:
      PLoS One. 2016 Aug 01;11(8):e0160398. (PMID: 27478893)
      Microb Cell Fact. 2020 Nov 18;19(1):212. (PMID: 33208159)
      J Tradit Chin Med. 2022 Jun;42(3):353-363. (PMID: 35610004)
    • Contributed Indexing:
      Keywords: grey relation analysis method; intestinal propulsion rate; slow transit constipation; spectrum-effect relationship; transdermal patches containing Cassia seed extract
    • Accession Number:
      0 (Plant Extracts)
      31C4KY9ESH (Nitric Oxide)
      0 (Drugs, Chinese Herbal)
    • Publication Date:
      Date Created: 20240506 Date Completed: 20240506 Latest Revision: 20240509
    • Publication Date:
      20240509
    • Accession Number:
      PMC11073935
    • Accession Number:
      10.12122/j.issn.1673-4254.2024.04.14
    • Accession Number:
      38708506