[Exercise promotes irisin expression to ameliorate renal injury in type 2 diabetic rats].

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
Read More Add to Saved list
  • Author(s): Zhou F;Zhou F;Zhou F; Guo Y; Guo Y; Guo Y; Chen N; Chen N; Chen N
  • Source:
    Nan fang yi ke da xue xue bao = Journal of Southern Medical University [Nan Fang Yi Ke Da Xue Xue Bao] 2024 Apr 20; Vol. 44 (4), pp. 675-681.
  • Publication Type:
    English Abstract; Journal Article
  • Language:
    Chinese
  • Additional Information
    • Source:
      Publisher: Nanfang yi ke da xue xue bao bian ji bu Country of Publication: China NLM ID: 101266132 Publication Model: Print Cited Medium: Print ISSN: 1673-4254 (Print) Linking ISSN: 16734254 NLM ISO Abbreviation: Nan Fang Yi Ke Da Xue Xue Bao Subsets: MEDLINE
    • Publication Information:
      Original Publication: Guangzhou : Nanfang yi ke da xue xue bao bian ji bu, 2005-
    • Subject Terms:
      Fibronectins*/metabolism ; Diabetes Mellitus, Type 2*/metabolism ; Rats, Sprague-Dawley* ; Physical Conditioning, Animal* ; Diabetes Mellitus, Experimental*/metabolism ; Kidney*/metabolism ; Sirtuin 1*/metabolism ; Autophagy*; Animals ; Male ; Rats ; Diabetic Nephropathies/metabolism ; Diabetic Nephropathies/therapy ; Beclin-1/metabolism ; Creatinine/blood ; Blood Urea Nitrogen ; Insulin ; Triglycerides/metabolism ; Triglycerides/blood ; Cholesterol/blood ; AMP-Activated Protein Kinases/metabolism
    • Abstract:
      Objective: To investigate the role of irisin in exercise-induced improvement of renal function in type 2 diabetic rats.
      Methods: Forty male SD rats aged 4-6 weeks were randomized into normal control group, type 2 diabetes mellitus model group, diabetic exercise (DE) group and diabetic irisin (DI) group ( n =8). The rats in DE group were trained with treadmill running for 8 weeks, and those in DI group were given scheduled irisin injections for 8 weeks. After the treatments, blood biochemical parameters of the rats were examined, and renal histopathology was observed with HE, Masson and PAS staining. Western blotting was used to detect the protein expression levels in the rats'kidneys.
      Results: The diabetic rats showed significantly increased levels of fasting insulin, total cholesterol, triglyceride, serum creatinine and blood urea nitrogen with lowered serum irisin level (all P < 0.05). Compared with those in DM group, total cholesterol, triglyceride, serum creatinine and blood urea nitrogen levels were decreased and serum irisin levels were increased in both DE and DI groups (all P < 0.05). The rats in DM group showed obvious structural disorders and collagen fiber deposition in the kidneys, which were significantly improved in DE group and DI group. Both regular exercises and irisin injections significantly ameliorated the reduction of FNDC5, LC3-II/I, Atg7, Beclin-1, p-AMPK, AMPK and SIRT1 protein expressions and lowered of p62 protein expression in the kidneys of the diabetic rats (all P < 0.05).
      Conclusion: Both exercise and exogenous irisin treatment improve nephropathy in type 2 diabetic rats possibly due to irisin-mediated activation of the AMPK/SIRT1 pathway in the kidneys to promote renal autophagy.
    • References:
      Cells. 2021 Sep 21;10(9):. (PMID: 34572148)
      Vet World. 2020 Oct;13(10):2191-2196. (PMID: 33281355)
      Pharmacol Res. 2022 Oct;184:106408. (PMID: 35988870)
      J Appl Physiol Respir Environ Exerc Physiol. 1979 Dec;47(6):1278-83. (PMID: 536299)
      Int J Mol Sci. 2020 Oct 14;21(20):. (PMID: 33066678)
      Diabetes Metab J. 2022 Mar;46(2):181-197. (PMID: 35385633)
      J Diabetes Complications. 2013 Jul-Aug;27(4):365-9. (PMID: 23619195)
      Kidney Int. 2022 Nov;102(5):974-989. (PMID: 36202661)
      Arch Physiol Biochem. 2023 Dec;129(4):943-950. (PMID: 33661722)
      Kidney Int. 2022 Aug;102(2):248-260. (PMID: 35661785)
      Cells. 2018 Nov 22;7(12):. (PMID: 30467302)
      Mol Cell Endocrinol. 2020 Jan 15;500:110628. (PMID: 31647955)
      Int Urol Nephrol. 2017 May;49(5):837-844. (PMID: 28035619)
      FASEB J. 2023 Oct;37(10):e23175. (PMID: 37742293)
      Peptides. 2019 Sep;119:170120. (PMID: 31351089)
      Nature. 2012 Jan 11;481(7382):463-8. (PMID: 22237023)
      Clin Chem Lab Med. 2018 Mar 28;56(4):525-548. (PMID: 29127759)
      J Diabetes Complications. 2014 Mar-Apr;28(2):208-13. (PMID: 24332937)
      Cell Biosci. 2020 Mar 30;10:51. (PMID: 32257109)
      Mol Cell. 2015 Dec 17;60(6):930-40. (PMID: 26626483)
      Cell Biol Int. 2022 Dec;46(12):2142-2157. (PMID: 36086947)
      Clin J Am Soc Nephrol. 2017 Dec 7;12(12):2032-2045. (PMID: 28522654)
      Int Urol Nephrol. 2020 Sep;52(9):1705-1712. (PMID: 32661628)
      Exp Gerontol. 2022 Oct 15;168:111934. (PMID: 36007721)
      Nat Rev Nephrol. 2019 Jun;15(6):327-345. (PMID: 30894700)
      Sci Rep. 2022 May 31;12(1):9062. (PMID: 35641586)
      Ann Clin Biochem. 2016 Jan;53(Pt 1):67-74. (PMID: 25814621)
    • Contributed Indexing:
      Keywords: AMPK/SIRT1 signaling pathway; autophagy; exercise; irisin; kidney; type 2 diabetic rat
    • Accession Number:
      0 (Fibronectins)
      EC 3.5.1.- (Sirtuin 1)
      0 (FNDC5 protein, rat)
      EC 3.5.1.- (Sirt1 protein, rat)
      0 (Beclin-1)
      AYI8EX34EU (Creatinine)
      0 (Insulin)
      0 (Triglycerides)
      97C5T2UQ7J (Cholesterol)
      EC 2.7.11.31 (AMP-Activated Protein Kinases)
    • Publication Date:
      Date Created: 20240506 Date Completed: 20240506 Latest Revision: 20240509
    • Publication Date:
      20240509
    • Accession Number:
      PMC11073942
    • Accession Number:
      10.12122/j.issn.1673-4254.2024.04.08
    • Accession Number:
      38708500