CircSSBP2 acts as a MiR-2400 sponge to promote intramuscular preadipocyte proliferation by regulating NDRG1.

Publisher: Springer-Verlag Country of Publication: Germany NLM ID: 101093320 Publication Model: Electronic Cited Medium: Internet ISSN: 1617-4623 (Electronic) Linking ISSN: 16174623 NLM ISO Abbreviation: Mol Genet Genomics Subsets: MEDLINE

Original Publication: Berlin : Springer-Verlag, c2001-

MicroRNAs*/genetics ; MicroRNAs*/metabolism ; Cell Proliferation*/genetics ; Adipocytes*/metabolism ; Adipocytes*/cytology ; Intracellular Signaling Peptides and Proteins*/genetics ; Intracellular Signaling Peptides and Proteins*/metabolism ; Cell Cycle Proteins*/genetics ; Cell Cycle Proteins*/metabolism ; Adipogenesis*/genetics; Animals ; Cattle ; RNA, Circular/genetics ; Gene Expression Regulation

Intramuscular fat (IMF) is a critical factor in beef quality. IMF is mainly distributed between muscle fibres and its accumulation can affect the marbling and meat quality of beef. IMF formation and deposition is a complex process and in recent years a group of non-coding RNAs (ncRNAs), known as circRNAs, have been discovered to play an important role in regulating intramuscular fat deposition. CircRNAs form a covalent loop structure after reverse splicing of precursor mRNAs. They can act by adsorbing miRNAs, thereby reducing their repressive effects on downstream target genes. Based on high-throughput sequencing of circRNAs in intramuscular fat of Qinchuan and Japanese black cattle, we identified a novel circSSBP2 that is differentially expressed between the two species and associated with adipogenesis. We show that circSSBP2 knockdown promotes bovine intramuscular preadipocyte proliferation, whereas overexpression inhibits bovine intramuscular preadipocyte proliferation. We also show that circSSBP2 can act as a molecular sponge for miR-2400 and that miR-2400 overexpression promotes bovine intramuscular preadipocyte proliferation. Furthermore, N-myc downstream-regulated gene 1 (NDRG1) was identified as a direct target gene of miR-2400, and NDRG1 interference promoted the proliferation of bovine intramuscular preadipocytes. In conclusion, our results suggest that circSSBP2 inhibits the proliferation of bovine intramuscular preadipocytes by regulating the miR-2400/NDRG1 axis.
(© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)

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2019BEF02004 This study was funded by the National Beef and Yak Industrial Technology System Project (CARS-37), National Natural Science Foundation of China (31972994), Ningxia Hui Autonomous Region Key Research and Development Project (2019BEF02004), and Shaanxi Agric

Keywords: Bovine; Intramuscular fat; NDRG1; Proliferation; circSSBP2; miR-2400

0 (MicroRNAs)
0 (N-myc downstream-regulated gene 1 protein)
0 (Intracellular Signaling Peptides and Proteins)
0 (Cell Cycle Proteins)
0 (RNA, Circular)

Date Created: 20240503 Date Completed: 20240503 Latest Revision: 20240523

20240523

10.1007/s00438-024-02138-1

38700639