Calpain 8 as a potential biomarker regulates the progression of pancreatic cancer via EMT and AKT/ERK pathway.

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  • Additional Information
    • Source:
      Publisher: Elsevier Country of Publication: Netherlands NLM ID: 101475056 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1876-7737 (Electronic) Linking ISSN: 18743919 NLM ISO Abbreviation: J Proteomics Subsets: MEDLINE
    • Publication Information:
      Original Publication: Amsterdam : Elsevier
    • Subject Terms:
      Calpain*/metabolism ; Pancreatic Neoplasms*/pathology ; Pancreatic Neoplasms*/metabolism ; Pancreatic Neoplasms*/genetics ; Biomarkers, Tumor*/metabolism ; Biomarkers, Tumor*/genetics ; Proto-Oncogene Proteins c-akt*/metabolism ; Epithelial-Mesenchymal Transition* ; MAP Kinase Signaling System*; Humans ; Male ; Cell Line, Tumor ; Female ; Disease Progression ; Gene Expression Regulation, Neoplastic ; Middle Aged ; Cell Proliferation ; Prognosis ; Cell Movement
    • Abstract:
      Calpain is a non-lysozyme, calcium-dependent intracellular cysteine protease that has been shown to play a role in tumor proliferation, survival, migration, invasion, and apoptosis. Dysregulation of calpain expression is closely related to tumorigenesis. However, the role of calpain-8 (CAPN8), as a member of the calpain family, in pancreatic cancer (PC) is remains unclear. In elucidating the mechanism of CAPN8 in PC, a comprehensive bioinformatics analysis and in vitro experiments were conducted. The TCGA database was used to explore the expression level of CAPN8, and the results in PC tissues and cell lines were verified. Then, the correlation between CAPN8 and clinicopathological features was analyzed. Additionaly, promoter methylation, immune infiltration, and GO/KEGG enrichment analyses were performed. Lastly, the molecular mechanism of CAPN8 in PC was investigated by using cell counting kit (CCK) 8, transwell, wound healing, Western blot assays, and so on. Results indicate that CAPN8 was highly expressed in PC and correlated with poor prognosis and advanced TNM stage. In addition, a low level of immune infiltration was closely associated with the high expression level of CAPN8. Based on these findings, we hypothesized that CAPN8 is a potential biomarker that regulates progression of PC via EMT and the AKT/ERK pathway. SIGNIFICANCE: Through comprehensive biological information and in vitro experiments, CAPN8 has been confirmed to play an important role in regulating pancreatic cancer (PC) proliferation, migration and invasion. CAPN8 is found to be closely related to the diagnosis, survival and prognosis of PC. Above all, CAPN8 may be a potential biomarker for the diagnosis and prognosis of PC.
      Competing Interests: Declaration of competing interest The authors declare that they have no competing interests.
      (Copyright © 2023. Published by Elsevier B.V.)
    • Contributed Indexing:
      Keywords: CAPN8; EMT; Invasion; Migration; Pancreatic cancer; Prognosis
    • Accession Number:
      EC 3.4.22.- (Calpain)
      0 (Biomarkers, Tumor)
      EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
    • Publication Date:
      Date Created: 20240502 Date Completed: 20240526 Latest Revision: 20240530
    • Publication Date:
      20240530
    • Accession Number:
      10.1016/j.jprot.2024.105182
    • Accession Number:
      38697284