A role for a Trypanosoma brucei cytosine RNA methyltransferase homolog in ribosomal RNA processing.

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
Read Online Read More Add to Saved list
  • Additional Information
    • Source:
      Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE
    • Publication Information:
      Original Publication: San Francisco, CA : Public Library of Science
    • Subject Terms:
      Trypanosoma brucei brucei*/genetics ; Trypanosoma brucei brucei*/enzymology ; Trypanosoma brucei brucei*/metabolism ; RNA, Ribosomal*/metabolism ; RNA, Ribosomal*/genetics ; RNA Processing, Post-Transcriptional*; Methyltransferases/metabolism ; Methyltransferases/genetics ; Protozoan Proteins/metabolism ; Protozoan Proteins/genetics ; RNA, Protozoan/metabolism ; RNA, Protozoan/genetics ; RNA Interference ; Methylation
    • Abstract:
      In Trypanosoma brucei, gene expression is primarily regulated posttranscriptionally making RNA metabolism critical. T. brucei has an epitranscriptome containing modified RNA bases. Yet, the identity of the enzymes catalyzing modified RNA base addition and the functions of the enzymes and modifications remain unclear. Homology searches indicate the presence of numerous T. brucei cytosine RNA methyltransferase homologs. One such homolog, TbNop2 was studied in detail. TbNop2 contains the six highly conserved motifs found in cytosine RNA methyltransferases and is evolutionarily related to the Nop2 protein family required for rRNA modification and processing. RNAi experiments targeting TbNop2 resulted in reduced levels of TbNop2 RNA and protein, and a cessation of parasite growth. Next generation sequencing of bisulfite-treated RNA (BS-seq) detected the presence of two methylation sites in the large rRNA; yet TbNop2 RNAi did not result in a significant reduction of methylation. However, TbNop2 RNAi resulted in the retention of 28S internal transcribed spacer RNAs, indicating a role for TbNop2 in rRNA processing.
      Competing Interests: The authors have declared that no competing interests exist.
      (Copyright: © 2024 Militello et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
    • References:
      Proc Natl Acad Sci U S A. 2000 Jul 18;97(15):8263-5. (PMID: 10899996)
      RNA Biol. 2020 Jul;17(7):1018-1039. (PMID: 32250712)
      Trends Parasitol. 2017 Feb;33(2):80-82. (PMID: 27863903)
      Cell. 2017 Jun 15;169(7):1187-1200. (PMID: 28622506)
      Nucleic Acids Res. 2018 Oct 12;46(18):9289-9298. (PMID: 30202881)
      RNA. 2002 Feb;8(2):237-46. (PMID: 11911368)
      Nucleic Acids Res. 2022 Oct 14;50(18):10695-10716. (PMID: 36161484)
      EMBO J. 2021 Feb 1;40(3):e105977. (PMID: 33470439)
      Cell Res. 2018 Apr;28(4):395-404. (PMID: 29463900)
      Nat Struct Mol Biol. 2012 Sep;19(9):900-5. (PMID: 22885326)
      PLoS One. 2015 Jul 21;10(7):e0133321. (PMID: 26196125)
      Nucleic Acids Res. 2012 Jun;40(11):5023-33. (PMID: 22344696)
      Mol Cell. 2006 Jan 6;21(1):87-96. (PMID: 16387656)
      RNA. 1999 Aug;5(8):1105-18. (PMID: 10445884)
      Mol Biochem Parasitol. 2007 Jun;153(2):220-3. (PMID: 17408766)
      Genomics. 2009 Apr;93(4):350-7. (PMID: 19135144)
      Nucleic Acids Res. 2013 Oct;41(19):9062-76. (PMID: 23913415)
      Nucleic Acids Res. 2018 Jul 2;46(W1):W537-W544. (PMID: 29790989)
      Genome Res. 2011 Jun;21(6):915-24. (PMID: 21363968)
      Nature. 2022 Apr;604(7905):362-370. (PMID: 35355019)
      Nucleic Acids Res. 2010 Jan;38(Database issue):D457-62. (PMID: 19843604)
      Nucleic Acids Res. 2022 Jan 25;50(2):1033-1051. (PMID: 34928368)
      J Mol Biol. 2000 Sep 8;302(1):205-17. (PMID: 10964570)
      Genes (Basel). 2019 Jan 30;10(2):. (PMID: 30704115)
      J Biol Chem. 1992 May 15;267(14):9805-15. (PMID: 1349605)
      Front Genet. 2018 Jul 12;9:243. (PMID: 30050561)
      Wiley Interdiscip Rev RNA. 2019 Jan;10(1):e1510. (PMID: 30311405)
      Proc Natl Acad Sci U S A. 1992 Mar 1;89(5):1827-31. (PMID: 1542678)
      Mol Biochem Parasitol. 2002 Nov-Dec;125(1-2):211-6. (PMID: 12467990)
      PLoS Genet. 2013 Jun;9(6):e1003602. (PMID: 23825970)
      Open Biol. 2019 Jun 28;9(6):190072. (PMID: 31164043)
      RNA. 2014 Oct;20(10):1632-44. (PMID: 25125595)
      J Protozool. 1977 May;24(2):325-9. (PMID: 881656)
      Nucleic Acids Res. 2009 Feb;37(2):e12. (PMID: 19059995)
      Mol Biochem Parasitol. 1999 Mar 15;99(1):89-101. (PMID: 10215027)
      Nucleic Acids Res. 2001 Dec 15;29(24):5216-25. (PMID: 11812855)
      Trends Parasitol. 2019 Oct;35(10):778-794. (PMID: 31473096)
      Biochemistry. 2002 Sep 17;41(37):11218-25. (PMID: 12220187)
      Nature. 2017 Feb 22;542(7642):494-497. (PMID: 28230119)
      Neuropsychopharmacology. 2013 Jan;38(1):23-38. (PMID: 22781841)
      Genes Dev. 2010 Aug 1;24(15):1590-5. (PMID: 20679393)
      Mol Biochem Parasitol. 2015 Mar-Apr;200(1-2):30-40. (PMID: 25736427)
      Crit Rev Biochem Mol Biol. 2020 Dec;55(6):525-540. (PMID: 32933330)
      Genome Biol. 2012 Oct 03;13(10):R87. (PMID: 23034086)
      Genome Biol. 2012 Oct 31;13(10):175. (PMID: 23113984)
      Nat Chem Biol. 2011 Oct 16;7(12):885-7. (PMID: 22002720)
      Sci Rep. 2016 May 04;6:25296. (PMID: 27142987)
      Trends Parasitol. 2011 Oct;27(10):434-41. (PMID: 21737348)
      Nucleic Acids Res. 1999 Aug 1;27(15):3138-45. (PMID: 10454610)
      Cold Spring Harb Protoc. 2010 Oct 01;2010(10):pdb.prot5505. (PMID: 20889702)
      Mol Biol Evol. 2021 Jun 25;38(7):3022-3027. (PMID: 33892491)
      Nucleic Acids Res. 2001 Jul 15;29(14):2927-37. (PMID: 11452018)
      Int J Mol Sci. 2022 Nov 10;23(22):. (PMID: 36430347)
      Mol Biochem Parasitol. 2014 Feb;193(2):122-6. (PMID: 24389163)
      Cell Res. 2017 May;27(5):606-625. (PMID: 28418038)
      Methods. 2001 Dec;25(4):402-8. (PMID: 11846609)
      Nat Commun. 2015 Jan 30;6:6158. (PMID: 25635753)
      Bioinformatics. 2011 Jun 1;27(11):1571-2. (PMID: 21493656)
      Mol Cell Biol. 1997 Jan;17(1):378-88. (PMID: 8972218)
      Trends Biochem Sci. 2015 Oct;40(10):560-575. (PMID: 26410597)
      Mol Microbiol. 2021 May;115(5):942-958. (PMID: 33513291)
      Open Biol. 2015 Jan;5(1):140197. (PMID: 25567099)
      Nucleic Acids Res. 2019 Mar 18;47(5):2609-2629. (PMID: 30605535)
      Nat Rev Mol Cell Biol. 2021 Jun;22(6):375-392. (PMID: 33658722)
      Proc Natl Acad Sci U S A. 2022 Oct 18;119(42):e2123338119. (PMID: 36240321)
      BMC Biol. 2020 Apr 15;18(1):40. (PMID: 32293435)
    • Grant Information:
      R15 AI133428 United States AI NIAID NIH HHS
    • Accession Number:
      0 (RNA, Ribosomal)
      EC 2.1.1.- (Methyltransferases)
      0 (Protozoan Proteins)
      0 (RNA, Protozoan)
    • Publication Date:
      Date Created: 20240425 Date Completed: 20240425 Latest Revision: 20240428
    • Publication Date:
      20250114
    • Accession Number:
      PMC11045063
    • Accession Number:
      10.1371/journal.pone.0298521
    • Accession Number:
      38662801