Birefringence mapping of biological tissues based on polarization sensitive non-interferometric quantitative phase imaging technique.

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  • Additional Information
    • Source:
      Publisher: Elsevier Country of Publication: Netherlands NLM ID: 101226123 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-1597 (Electronic) Linking ISSN: 15721000 NLM ISO Abbreviation: Photodiagnosis Photodyn Ther Subsets: MEDLINE
    • Publication Information:
      Original Publication: Amsterdam ; Boston : Elsevier, c2004-
    • Subject Terms:
      Microscopy, Polarization*/methods ; Mouth Neoplasms*/diagnostic imaging; Birefringence ; Humans ; Algorithms ; Refractometry/methods ; Quantitative Phase Imaging
    • Abstract:
      Objective: Oral cancer is a leading cause of mortality globally, particularly affecting developing regions where oral hygiene is often overlooked. The optical properties of tissues are vital for diagnostics, with polarization imaging emerging as a label-free, contrast-enhancing technique widely employed in medical and scientific research over past few decades.
      Materials and Methods: We present a novel polarization sensitive quantitative phase imaging of biological tissues by incorporating the conventional polarization microscope and transport of intensity equation-based phase retrieval algorithm. This integration provides access to the birefringence mapping of biological tissues. The inherent optical anisotropy in biological tissues induces the polarization dependent refractive index variations which can provide the detailed insights into the birefringence characteristics of their extracellular constituents. Experimental investigations were conducted on both normal and cancerous oral tissue samples by recording a set of three polarization intensity images for each case with a step size of 2 μm.
      Results: A noteworthy increment in birefringence quantification was observed in cancerous as compared to the normal tissues, attributed to the proliferation of abnormal cells during cancer progression. The mean birefringence values were calculated for both normal and cancerous tissues, revealing a significant increase in birefringence of cancerous tissues (2.1 ± 0.2) × 10 -2 compared to normal tissues (0.8 ± 0.2) × 10 -2 . Data were collected from 8 patients in each group under identical experimental conditions.
      Conclusion: This polarization sensitive non-interferometric optical approach demonstrated effective discrimination between cancerous and normal tissues, with various parameters indicating elevated values in cancerous tissues.
      Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
      (Copyright © 2024. Published by Elsevier B.V.)
    • Contributed Indexing:
      Keywords: Anisotropy; Birefringence; Intensity; Phase; Polarization; Transport of intensity
    • Publication Date:
      Date Created: 20240420 Date Completed: 20240530 Latest Revision: 20240530
    • Publication Date:
      20240531
    • Accession Number:
      10.1016/j.pdpdt.2024.104094
    • Accession Number:
      38642728