Multi-locus sequence analysis of Anaplasma bovis in goats and ticks from Thailand, with the initial identification of an uncultured Anaplasma species closely related to Anaplasma phagocytophilum-like 1.

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  • Additional Information
    • Source:
      Publisher: Elsevier Science Ltd Country of Publication: England NLM ID: 7808924 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1878-1667 (Electronic) Linking ISSN: 01479571 NLM ISO Abbreviation: Comp Immunol Microbiol Infect Dis Subsets: MEDLINE
    • Publication Information:
      Publication: Exeter : Elsevier Science Ltd
      Original Publication: Oxford, Elmsford, N. Y., Pergamon Press.
    • Subject Terms:
      Goats* ; Anaplasma*/genetics ; Anaplasma*/isolation & purification ; Anaplasma*/classification ; Anaplasmosis*/microbiology ; Anaplasmosis*/epidemiology ; Goat Diseases*/microbiology ; Goat Diseases*/epidemiology ; Phylogeny* ; RNA, Ribosomal, 16S*/genetics ; Multilocus Sequence Typing*; Animals ; Thailand/epidemiology ; Anaplasma phagocytophilum/genetics ; Anaplasma phagocytophilum/isolation & purification ; Ticks/microbiology ; DNA, Bacterial/genetics
    • Abstract:
      Ticks and tick-borne pathogens (TTBP) pose a serious threat to animal and human health globally. Anaplasma bovis, an obligatory intracellular bacterium, is one of the more recent species of the Family Anaplasmaceae to be formally described. Owing to its diminutive size, microscopic detection presents a formidable challenge, leading to it being overlooked in laboratory settings lacking advanced equipment or resources, as observed in various regions, including Thailand. This study aimed to undertake a genetic analysis of A. bovis and determine its prevalence in goats and ticks utilizing three genetic markers (16S rRNA, gltA, groEL). A total of 601 goat blood and 118 tick samples were collected from 12 sampling sites throughout Thailand. Two tick species, Haemaphysalis bispinosa (n = 109), and Rhipicephalus microplus (n = 9) were identified. The results herein showed that 13.8 % (83/601) of goats at several farms and 5 % (1/20) of ticks were infected with A. bovis. Among infected ticks, A. bovis and an uncultured Anaplasma sp. which are closely related to A. phagocytophilum-like 1, were detected in each of H. bispinosa ticks. The remaining R. microplus ticks tested positive for the Anaplasma genus. A nucleotide sequence type network showed that A. bovis originated from Nan and Narathiwat were positioned within the same cluster and closely related to China isolates. This observation suggests the potential dispersal of A. bovis over considerable distances, likely facilitated by activities such as live animal trade or the transportation of infected ticks via migratory birds. The authors believe that the findings from this study will provide valuable information about TTBP in animals.
      Competing Interests: Declaration of Competing Interest The authors declare no conflicts of interest. We additionally affirm the originality of our work, stating that it has not been submitted elsewhere. All authors have thoroughly reviewed the final manuscript and concur with its content.
      (Copyright © 2024 Elsevier Ltd. All rights reserved.)
    • Contributed Indexing:
      Keywords: Anaplasma bovis; Goat; Thailand; Ticks; Uncultured Anaplasma sp.
    • Subject Terms:
      Anaplasma bovis
    • Publication Date:
      Date Created: 20240418 Date Completed: 20240519 Latest Revision: 20240528
    • Publication Date:
      20250114
    • Accession Number:
      10.1016/j.cimid.2024.102181
    • Accession Number:
      38636298