Role of the 3-mercaptopyruvate sulfurtransferase in colon/colorectal cancers.

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  • Additional Information
    • Source:
      Publisher: Elsevier Country of Publication: Germany NLM ID: 7906240 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1618-1298 (Electronic) Linking ISSN: 01719335 NLM ISO Abbreviation: Eur J Cell Biol Subsets: MEDLINE
    • Publication Information:
      Publication: Jena, Germany : Elsevier
      Original Publication: Stuttgart : Wissenschaftliche Verlagsgesellschaft, <1979-1997>
    • Subject Terms:
      Colorectal Neoplasms*/metabolism ; Colorectal Neoplasms*/pathology ; Colorectal Neoplasms*/enzymology ; Colorectal Neoplasms*/genetics ; Sulfurtransferases*/metabolism ; Sulfurtransferases*/genetics; Humans ; Colonic Neoplasms/metabolism ; Colonic Neoplasms/pathology ; Colonic Neoplasms/genetics ; Colonic Neoplasms/enzymology ; Apoptosis ; Cell Proliferation ; Mitochondria/metabolism ; HCT116 Cells ; Cell Movement ; Membrane Potential, Mitochondrial ; Cell Line, Tumor ; Dynamins/metabolism ; Dynamins/genetics
    • Abstract:
      The 3-mercaptopyruvate sulfurtransferase (MPST) is a protein persulfidase, occurring mainly in mitochondria. Although function of this protein in cancer cells has been already studied, no clear outcome can be postulated up to now. Therefore, we focused on the determination of function of MPST in colon (HCT116 cells)/colorectal (DLD1 cells) cancers. In silico analysis revealed that in gastrointestinal cancers, MPST together with its binding partners can be either of a high risk or might have a protective effect. Silencing of MPST gene resulted in decreased ATP, while acetyl-CoA levels were elevated. Increased apoptosis was detected in cells with silenced MPST gene, which was accompanied by decrease in mitochondrial membrane potential, but no changes in IP 3 receptor's protein. Mitochondria underwent activation of fission and elevated DRP1 expression after MPST silencing. Proliferation and migration of DLD1 and HCT116 cells were markedly affected, showing the importance of MPST protein in colon/colorectal cancer development.
      Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
      (Copyright © 2024 The Authors. Published by Elsevier GmbH.. All rights reserved.)
    • Contributed Indexing:
      Keywords: 3- mercaptopyruvate sulfurtransferase; Colon/colorectal cancer; Fission; Mitochondria
    • Accession Number:
      EC 2.8.1.- (Sulfurtransferases)
      EC 2.8.1.2 (3-mercaptopyruvate sulphurtransferase)
      EC 3.6.5.5 (Dynamins)
      EC 3.6.5.5 (DNM1L protein, human)
    • Publication Date:
      Date Created: 20240417 Date Completed: 20240627 Latest Revision: 20240719
    • Publication Date:
      20240719
    • Accession Number:
      10.1016/j.ejcb.2024.151415
    • Accession Number:
      38631098