Characteristics of spatial protein expression in the mouse cochlear sensory epithelia: Implications for age-related hearing loss.

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  • Additional Information
    • Source:
      Publisher: Elsevier/North-Holland Biomedical Press Country of Publication: Netherlands NLM ID: 7900445 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1878-5891 (Electronic) Linking ISSN: 03785955 NLM ISO Abbreviation: Hear Res Subsets: MEDLINE
    • Publication Information:
      Original Publication: Amsterdam, Elsevier/North-Holland Biomedical Press.
    • Subject Terms:
      Mice, Inbred C57BL* ; Proteomics* ; Cochlea*/metabolism ; Cochlea*/pathology ; Presbycusis*/metabolism ; Presbycusis*/pathology ; Presbycusis*/physiopathology ; Presbycusis*/genetics; Animals ; Age Factors ; Hair Cells, Auditory/metabolism ; Hair Cells, Auditory/pathology ; Aging/metabolism ; Aging/pathology ; Disease Models, Animal ; Hearing ; Epithelium/metabolism ; Male ; Mice
    • Abstract:
      Hair cells in the cochlear sensory epithelia serve as mechanosensory receptors, converting sound into neuronal signals. The basal sensory epithelia are responsible for transducing high-frequency sounds, while the apex handles low-frequency sounds. Age-related hearing loss predominantly affects hearing at high frequencies and is indicative of damage to the basal sensory epithelia. However, the precise mechanism underlying this site-selective injury remains unclear. In this study, we employed a microscale proteomics approach to examine and compare protein expression in different regions of the cochlear sensory epithelia (upper half and lower half) in 1.5-month-old (normal hearing) and 6-month-old (severe high-frequency hearing loss without hair cell loss) C57BL/6J mice. A total of 2,386 proteins were detected, and no significant differences in protein expression were detected in the upper half of the cochlear sensory epithelia between the two age groups. The expression of 20 proteins in the lower half of the cochlear sensory epithelia significantly differed between the two age groups (e.g., MATN1, MATN4, and AQP1). Moreover, there were 311 and 226 differentially expressed proteins between the upper and lower halves of the cochlear sensory epithelia in 1.5-month-old and 6-month-old mice, respectively. The expression levels of selected proteins were validated by Western blotting. These findings suggest that the spatial differences in protein expression within the cochlear sensory epithelia may play a role in determining the susceptibility of cells at different sites of the cochlea to age-related damage.
      (Copyright © 2024 Elsevier B.V. All rights reserved.)
    • Contributed Indexing:
      Keywords: Age-related hearing loss; Cochlea; Gradient; Proteomics; Sensory epithelia; Tonotopy
    • Publication Date:
      Date Created: 20240407 Date Completed: 20240427 Latest Revision: 20240923
    • Publication Date:
      20240924
    • Accession Number:
      10.1016/j.heares.2024.109006
    • Accession Number:
      38583350