Amygdala-Targeted Relief of Neuropathic Pain: Efficacy of Repetitive Transcranial Magnetic Stimulation in NLRP3 Pathway Suppression.

Publisher: Humana Press Country of Publication: United States NLM ID: 8900963 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1559-1182 (Electronic) Linking ISSN: 08937648 NLM ISO Abbreviation: Mol Neurobiol Subsets: MEDLINE

Original Publication: Clifton, NJ : Humana Press, c1987-

Amygdala*/metabolism ; Neuralgia*/therapy ; Neuralgia*/metabolism ; NLR Family, Pyrin Domain-Containing 3 Protein*/metabolism ; Signal Transduction* ; Transcranial Magnetic Stimulation*/methods; Animals ; Male ; Rats ; Integrin alphaVbeta3/metabolism ; Rats, Sprague-Dawley

This study investigates the effectiveness of repetitive transcranial magnetic stimulation (rTMS) as a nonpharmacological approach to treating neuropathic pain (NP), a major challenge in clinical research. Conducted on male Sprague-Dawley rats with NP induced through chronic constriction injury of the sciatic nerve, the research assessed pain behaviors and the impact of rTMS on molecular interactions within the amygdala. Through a comprehensive analysis involving Mechanical Withdrawal Threshold (MWT), Thermal Withdrawal Latency (TWL), RNA transcriptome sequencing, RT-qPCR, Western blotting, immunofluorescence staining, and Co-Immunoprecipitation (Co-IP), the study focused on the expression and interaction of integrin αvβ3 and its receptor P2X7R. Findings reveal that rTMS significantly influences the expression of integrin αvβ3 in NP models, suggesting an inhibition of the NP-associated NLRP3 inflammatory pathway through the disruption of integrin αvβ3-P2X7R interactions. These outcomes highlight the potential of rTMS in alleviating NP by targeting molecular interactions within the amygdala, offering a promising therapeutic avenue for managing NP.
(© 2024. The Author(s).)

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82360164 Natural Science Foundation of China; 23A0729 Project of Hunan Provincial Department of Education; 202218015847, 20231214 Project of Hunan Provincial Health Commission; 2023SK4014 Natural Science Foundation of Hunan Province

Keywords: Amygdala; Integrin αvβ3; NLRP3 Inflammatory Pathway; Neuropathic pain; P2 × 7R; Repetitive Transcranial Magnetic Stimulation

0 (Integrin alphaVbeta3)
0 (NLR Family, Pyrin Domain-Containing 3 Protein)

Date Created: 20240404 Date Completed: 20241022 Latest Revision: 20241123

20241123

PMC11496354

10.1007/s12035-024-04087-7

38573415