Phorbol ester-induced lymphocyte adherence: selective action on NK cells.

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  • Author(s): Argov S; Hebdon M; Cuatrecasas P; Koren HS
  • Source:
    Journal of immunology (Baltimore, Md. : 1950) [J Immunol] 1985 Apr; Vol. 134 (4), pp. 2215-22.
  • Publication Type:
    Journal Article; Research Support, U.S. Gov't, P.H.S.
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: American Association of Immunologists Country of Publication: United States NLM ID: 2985117R Publication Model: Print Cited Medium: Print ISSN: 0022-1767 (Print) Linking ISSN: 00221767 NLM ISO Abbreviation: J Immunol Subsets: MEDLINE
    • Publication Information:
      Publication: Bethesda, MD : American Association of Immunologists
      Original Publication: Baltimore : Williams & Wilkins, c1950-
    • Subject Terms:
      Cell Adhesion/*drug effects ; Cytotoxicity, Immunologic/*drug effects ; Killer Cells, Natural/*physiology ; Phorbol Esters/*pharmacology ; Phorbols/*pharmacology; Antibody-Dependent Cell Cytotoxicity/drug effects ; Cell Line ; Cell Separation ; Humans ; Interferon Type I/pharmacology ; Killer Cells, Natural/cytology ; Killer Cells, Natural/immunology ; Kinetics ; Leukemia, Erythroblastic, Acute/immunology ; Lymphocyte Activation/drug effects ; Phenotype ; Phorbol 12,13-Dibutyrate
    • Abstract:
      Treatment of human peripheral blood lymphocytes (PBL) with phorbol dibutyrate (PDBU) for 20 to 45 min at 37 degrees C induces adherence of 5 to 30% of the cells to plastic. The adherent cells (pAd) were highly enriched in NK cells on the basis of the following findings: 1) they exhibit high NK and ADCC activity but do not lyse the NK-resistant cell line, Daudi; 2) cytotoxic activity is enhanced by pretreatment with interferon-alpha (IFN-alpha); 3) the surface markers of these cells, as determined with monoclonal antibodies, are consistent with NK cells; and 4) they are enriched with cells morphologically similar to large granular lymphocytes. Conversely, the PDBU-nonadherent cells were substantially depleted of NK cells. The fact that the pAd cells do not lyse the Daudi line and that their NK activity can be further augmented by IFN-alpha would suggest that the pAd are enriched for NK cells rather than changed in their characteristics as a result of the separation procedure. Moreover, no consistent and appreciable modulation of NK activity induced by PDBU was observed. This report therefore demonstrates that PDBU selectively induces NK adherence of NK cells, which may have practical as well as biological implications.
    • Grant Information:
      CA 23354 United States CA NCI NIH HHS; CA 29589 United States CA NCI NIH HHS
    • Accession Number:
      0 (Interferon Type I)
      0 (Phorbol Esters)
      0 (Phorbols)
      37558-16-0 (Phorbol 12,13-Dibutyrate)
    • Publication Date:
      Date Created: 19850401 Date Completed: 19850423 Latest Revision: 20071114
    • Publication Date:
      20240829
    • Accession Number:
      3855930