Bioprospecting of Chlamydomonas reinhardtii for boosting biofuel-related products production based on novel aggregation-induced emission active extracellular polymeric substances nanoprobes.

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  • Additional Information
    • Source:
      Publisher: Elsevier Applied Science Country of Publication: England NLM ID: 9889523 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-2976 (Electronic) Linking ISSN: 09608524 NLM ISO Abbreviation: Bioresour Technol Subsets: MEDLINE
    • Publication Information:
      Original Publication: Barking, Essex, England : New York, N.Y. : Elsevier Applied Science ; Elsevier Science Pub. Co., 1991-
    • Subject Terms:
      Chlamydomonas reinhardtii*/genetics ; Chlamydomonas reinhardtii*/metabolism ; Microalgae*/metabolism; Biofuels ; Extracellular Polymeric Substance Matrix/metabolism ; Bioprospecting ; Chlorophyll/metabolism
    • Abstract:
      Biofuel production from microalgae has been greatly restricted by low biomass productivity and long-term photosynthetic efficacy. Here, a novel strategy for selecting high-growing, stress-resistant algal strains with high photosynthetic capacity was proposed based on biocompatible extracellular polymeric substances (EPS) probes with aggregation-induced emission (AIE) properties. Specifically, AIE active EPS probes were synthesized for in-situ long-term monitoring of the EPS productivity at different algal growth stages. By coupling the AIE-based fluorescent techniques, algal cells were classified into four diverse populations based on their chlorophyll and EPS signals. Mechanistic studies on the sorted algal cells revealed their remarkable stress resistance and high expression of cell division, biopolymer production and photosynthesis-related genes. The sorted and subcultured algal cells consistently exhibited relatively higher growth rates and photosynthetic capacities, resulting in an increased (1.2 to 1.8-fold) algal biomass production, chlorophyll, and lipids. This study can potentially open new strategies to boost microalgal-based biofuel production.
      Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
      (Copyright © 2024 Elsevier Ltd. All rights reserved.)
    • Contributed Indexing:
      Keywords: AIE-active EPS probes; Cell sorting and subculturing; Chlamydomonas reinhardtii; Fluorescence-activated cell sorting
    • Accession Number:
      0 (Biofuels)
      1406-65-1 (Chlorophyll)
    • Publication Date:
      Date Created: 20240328 Date Completed: 20240412 Latest Revision: 20240412
    • Publication Date:
      20240412
    • Accession Number:
      10.1016/j.biortech.2024.130636
    • Accession Number:
      38548032