The accessible chromatin landscape of lipopolysaccharide-induced systemic inflammatory response identifying epigenome signatures and transcription regulatory networks in chickens.

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  • Additional Information
    • Source:
      Publisher: Elsevier Country of Publication: Netherlands NLM ID: 7909578 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0003 (Electronic) Linking ISSN: 01418130 NLM ISO Abbreviation: Int J Biol Macromol Subsets: MEDLINE
    • Publication Information:
      Publication: Amsterdam : Elsevier
      Original Publication: Guildford, Eng., IPC Science and Technology Press.
    • Subject Terms:
      Chickens* ; Lipopolysaccharides* ; Gene Regulatory Networks*/drug effects ; Chromatin*/genetics ; Chromatin*/metabolism; Animals ; Epigenome ; Inflammation/genetics ; Inflammation/chemically induced ; Gene Expression Regulation/drug effects ; Cytokines/metabolism ; Cytokines/genetics ; Epigenesis, Genetic/drug effects ; Chromatin Assembly and Disassembly/drug effects ; Transcription Factors/genetics ; Transcription Factors/metabolism
    • Abstract:
      Lipopolysaccharide (LPS) can induce systemic inflammatory response (SIR) in animals. Understanding the regulatory mechanism of SIR and therapies to ensure healthy growth is urgently needed. Chromatin remodeling plays a crucial role in the expression of genes involved in immune diseases. In the present study, the ATAC-seq analysis revealed 3491 differential open chromatin sites in the spleen of chicks with SIR induced by LPS challenge, and we presented the motifs on these sites and the associated transcription factors. The regulatory network was presented by combining the differential open chromatin data with the mRNAs and exploded cytokines. Interestingly, the LPS challenge could regulate the mRNA expression of 202 genes through chromatin reprogramming, including critical genes such as TLE1 and JUN, which regulate signaling pathways such as I-κB kinase/NF-κB, Toll-like receptor, and downstream cytokine genes. Furthermore, dietary daidzein could inhibit DNA topoisomerase II, which reprograms the spatial conformation of chromatin in the inflammatory response and attenuates SIR. In conclusion, we successfully identified key genes directly regulated by chromatin reprogramming in SIR and demonstrated the chromatin epigenome signatures and transcriptional regulatory network, which provides an important reference for further research on avian epigenetics. There is great potential for alleviating SIR using dietary daidzein.
      Competing Interests: Declaration of competing interest The authors have declared that no competing interests exist.
      (Copyright © 2024 Elsevier B.V. All rights reserved.)
    • Contributed Indexing:
      Keywords: Accessible chromatin landscape; Lipopolysaccharide; Systemic inflammatory response
    • Accession Number:
      0 (Lipopolysaccharides)
      0 (Chromatin)
      0 (Cytokines)
      0 (Transcription Factors)
    • Publication Date:
      Date Created: 20240328 Date Completed: 20240430 Latest Revision: 20240430
    • Publication Date:
      20240501
    • Accession Number:
      10.1016/j.ijbiomac.2024.131136
    • Accession Number:
      38547952