Lymphopenia associated with survivin and its downstream pathway in COVID-19 serving as a potential route in COVID-19 pathogenesis.

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  • Additional Information
    • Source:
      Publisher: Elsevier Country of Publication: Netherlands NLM ID: 101276222 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1898-4002 (Electronic) Linking ISSN: 18961126 NLM ISO Abbreviation: Adv Med Sci Subsets: MEDLINE
    • Publication Information:
      Publication: March, 2014- : Amsterdam : Elsevier
      Original Publication: Białystok, Poland : Medical University of Białystok, [2006]-
    • Subject Terms:
      Survivin*/metabolism ; COVID-19*/metabolism ; COVID-19*/virology ; Apoptosis* ; Lymphopenia*/metabolism ; SARS-CoV-2*/pathogenicity; Humans ; X-Linked Inhibitor of Apoptosis Protein/metabolism ; Male ; Female ; Leukocytes, Mononuclear/metabolism ; Middle Aged ; Adult ; Signal Transduction
    • Abstract:
      Purpose: Starting in 2019, coronavirus disease 2019 (COVID-19) caused an epidemic that was growing rapidly and has harmed millions of people globally. It has been demonstrated that survivin regulates lymphocyte survival, a main route involved in COVID-19 pathogenesis. Survivin belongs to the inhibitor of apoptosis protein (IAP) family, and its primary functions comprise regulating mitosis and inhibiting apoptosis. Since lower survivin expression has been shown to increase the sensitivity of lymphocytes to apoptotic induction, we looked into the function of survivin and its corresponding pathways in COVID-19 pathogenesis.
      Materials and Methods: The expression of survivin, X-linked inhibitor of apoptosis protein (XIAP), caspases 3, 7, 9, and poly (ADP-ribose) polymerase (PARP) was evaluated at both mRNA and protein levels in peripheral blood mononuclear cells (PBMCs) derived from healthy donors and patients with severe and moderate COVID-19 by qRT-PCR and Western blotting, respectively. Then, we enforced apoptosis to COVID-19 patient-derived lymphocytes, and the percent was assessed by flow cytometry.
      Results: Survivin and XIAP were less expressed in PBMCs derived from COVID-19 patients as apoptosis inhibitors than PARP, cleaved-PARP, caspase 9, and cleaved caspases 3 and 7, according to the results of real-time PCR and Western blot analysis. Additionally, according to the flow cytometry results, the down-regulation of survivin served as a potential factor in the lymphocyte depletion observed in patients with COVID-19.
      Conclusion: The role of survivin and its related pathway was first discovered in the development of COVID-19 and may serve as a potential prognostic and therapeutic target.
      Competing Interests: Declaration of competing interest The authors declare no conflict of interests.
      (Copyright © 2024 Medical University of Bialystok. Published by Elsevier B.V. All rights reserved.)
    • Contributed Indexing:
      Keywords: Apoptosis; Apoptotic pathway; COVID-19; Survivin
    • Accession Number:
      0 (Survivin)
      0 (BIRC5 protein, human)
      0 (X-Linked Inhibitor of Apoptosis Protein)
      0 (XIAP protein, human)
    • Publication Date:
      Date Created: 20240323 Date Completed: 20240502 Latest Revision: 20240523
    • Publication Date:
      20240523
    • Accession Number:
      10.1016/j.advms.2024.03.006
    • Accession Number:
      38521459