Preparation, Characterization and In vitro Evaluation of Insulin-PHBV Nanoparticles/Alginate Hydrogel Composite System for Prolonged Delivery of Insulin.

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  • Additional Information
    • Source:
      Publisher: Elsevier Country of Publication: United States NLM ID: 2985195R Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1520-6017 (Electronic) Linking ISSN: 00223549 NLM ISO Abbreviation: J Pharm Sci Subsets: MEDLINE
    • Publication Information:
      Publication: 2016- : New York, NY : Elsevier
      Original Publication: Easton, Pa., American Pharmaceutical Assn.
    • Subject Terms:
      Alginates*/chemistry ; Insulin*/administration & dosage ; Insulin*/chemistry ; Hydrogels*/chemistry ; Nanoparticles*/chemistry ; Cell Survival*/drug effects ; Polyesters*/chemistry ; Delayed-Action Preparations*/chemistry; Animals ; Mice ; Cell Line ; Glucuronic Acid/chemistry ; Hexuronic Acids/chemistry ; Drug Delivery Systems/methods ; Drug Carriers/chemistry ; Drug Liberation ; Hypoglycemic Agents/administration & dosage ; Hypoglycemic Agents/chemistry ; Hypoglycemic Agents/pharmacokinetics ; Hypoglycemic Agents/pharmacology ; Polyhydroxybutyrates
    • Abstract:
      Purpose: In the present study, biodegradable poly (3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) nanoparticles (NPs) containing insulin were loaded in sodium alginate/jeffamine (ALG/jeff) hydrogel for prolonged delivery of insulin. The main aim of this work was to fabricate an efficient insulin delivery system to improve patient adherence by decreasing the repetition of injections.
      Methods: Swelling and morphological properties and crosslinking efficiency of ALG/jeff hydrogel were assessed. The composite hydrogel was prepared by adding PHBV NPs to ALG/jeff hydrogel concurrently with crosslinking process. The morphology and loading capacity of composite hydrogel were analyzed.
      Results: Circular dichroism measurement demonstrated that insulin remains stable following fabrication process. The release profile exhibited 54.6 % insulin release from composite hydrogel within 31 days with minor initial burst release equated to nanoparticles and hydrogels. MTT cell viability analysis was performed by applying L-929 cell line and no cytotoxic effect was observed.
      Conclusions: Favorable results clearly introduced fabricated composite hydrogel as an excellent candidate for drug delivery systems and also paves the route for prolonged delivery systems of other proteins.
      Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
      (Copyright © 2024. Published by Elsevier Inc.)
    • Contributed Indexing:
      Keywords: Biocompatibility; Biodegradable polymer; Cell line; Composite hydrogel; Controlled release; Drug delivery system; Insulin; PHBV nanoparticles; Prolonged delivery; Sodium alginate/jeffamine hydrogel
    • Accession Number:
      0 (Alginates)
      0 (Insulin)
      0 (poly(3-hydroxybutyrate)-co-(3-hydroxyvalerate))
      0 (Hydrogels)
      0 (Polyesters)
      0 (Delayed-Action Preparations)
      8A5D83Q4RW (Glucuronic Acid)
      0 (Hexuronic Acids)
      0 (Drug Carriers)
      0 (Hypoglycemic Agents)
      0 (Polyhydroxybutyrates)
    • Publication Date:
      Date Created: 20240320 Date Completed: 20240828 Latest Revision: 20240911
    • Publication Date:
      20250114
    • Accession Number:
      10.1016/j.xphs.2024.03.010
    • Accession Number:
      38508339