A novel enrofloxacin-degrading fungus, Humicola sp. KC0924g, isolated from the rhizosphere sediment of the submerged macrophyte Vallisneria spiralis L.

Publisher: Springer Nature Country of Publication: Switzerland NLM ID: 9816585 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1618-1905 (Electronic) Linking ISSN: 11396709 NLM ISO Abbreviation: Int Microbiol Subsets: MEDLINE

Publication: 2018- : Switzerland AG : Springer Nature
Original Publication: Barcelona, Spain : Springer, c1998-

Rhizosphere* ; Enrofloxacin*/metabolism ; Anti-Bacterial Agents*/metabolism ; Anti-Bacterial Agents*/pharmacology ; Biodegradation, Environmental*; Geologic Sediments/microbiology ; Ascomycota/metabolism ; Ascomycota/genetics ; Ascomycota/classification ; Ascomycota/isolation & purification ; Phylogeny ; Metabolic Networks and Pathways/genetics ; Fluoroquinolones/metabolism

A novel enrofloxacin-degrading fungus was isolated from a rhizosphere sediment of the submerged macrophyte Vallisneria spiralis L.. The isolate, designated KC0924g, was identified as a member of the genus Humicola based on morphological characteristics and tandem conserved sequence analysis. The optimal temperature and pH for enrofloxacin degradation by strain KC0924g were 28 °C and 9.0, respectively. Under such condition, 98.2% of enrofloxacin with an initial concentration of 1 mg L ^-1 was degraded after 72 h of incubation, with nine possible degradation products identified. Four different metabolic pathways were proposed, which were initiated by cleavage of the piperazine moiety, hydroxylation of the aromatic ring, oxidative decarboxylation, or defluorination. In addition to enrofloxacin, strain KC0924g also degraded other fluoroquinolone antibiotics (ciprofloxacin, norfloxacin, and ofloxacin), malachite green (an illegal additive in aquaculture), and leucomalachite green. Pretreatment of cells of strain KC0924g with Cu ²⁺ accelerated ENR degradation. Furthermore, it was speculated that a flavin-dependent monooxygenase was involved in ENR degradation, based on the increased transcriptional levels of these two genes after Cu ²⁺ induction. This work enriches strain resources for enrofloxacin remediation and, more importantly, would facilitate studies on the molecular mechanism of ENR degradation with degradation-related transcriptome available.
Competing Interests: Declarations. Ethics approval and consent to participate: Not applicable. Consent for publication: All authors have read the manuscript and agreed to its publication. Competing interests: The authors declare no competing interests.
(© 2024. The Author(s), under exclusive licence to Springer Nature Switzerland AG.)

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2020-02-08-00-07-F01483 Shanghai Agriculture Applied Technology Development Program, China; 31900097 National Natural Science Foundation of China; 20ZR1450800 Natural Science Foundation of Shanghai

Keywords: Humicola sp.; Biodegradation; Comparative transcriptome analysis; Degradation pathway; Enrofloxacin

3DX3XEK1BN (Enrofloxacin)
0 (Anti-Bacterial Agents)
0 (Fluoroquinolones)

Date Created: 20240320 Date Completed: 20241202 Latest Revision: 20241202

20241204

10.1007/s10123-024-00513-x

38506947