Mercuric sulfide nanoparticles suppress the neurobehavioral functions of Caenorhabditis elegans through a Skp1-dependent mechanism.

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
Read More Add to Saved list
  • Author(s): Li L;Li L; Li Y; Li Y; Zeng K; Zeng K; Wang Q; Wang Q; Wang Q; Wang Q
  • Source:
    Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association [Food Chem Toxicol] 2024 Apr; Vol. 186, pp. 114576. Date of Electronic Publication: 2024 Mar 06.
  • Publication Type:
    Journal Article
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Elsevier Science Ltd Country of Publication: England NLM ID: 8207483 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-6351 (Electronic) Linking ISSN: 02786915 NLM ISO Abbreviation: Food Chem Toxicol Subsets: MEDLINE
    • Publication Information:
      Publication: Exeter : Elsevier Science Ltd
      Original Publication: Oxford ; New York : Pergamon Press, c1982-
    • Subject Terms:
      Mercury Compounds*/toxicity ; Nanoparticles*/toxicity ; Caenorhabditis elegans Proteins*/metabolism; Animals ; Caenorhabditis elegans/metabolism ; S-Phase Kinase-Associated Proteins/genetics
    • Abstract:
      Cinnabar is the naturally occurring mercuric sulfide (HgS) and concerns about its safety have been grown. However, the molecular mechanism of HgS-related neurotoxicity remains unclear. S-phase kinase-associated protein 1 (Skp1), identified as the target protein of HgS, plays a crucial role in the development of neurological diseases. This study aims to investigate the neurotoxic effects and molecular mechanism of HgS based on Skp1 using the Caenorhabditis elegans (C. elegans) model. We prepared the HgS nanoparticles and conducted a comparative analysis of neurobehavioral differences in both wild-type C. elegans (N2) and a transgenic strain of C. elegans (VC1241) with a knockout of the SKP1 homologous gene after exposure to HgS nanoparticles. Our results showed that HgS nanoparticles could suppress locomotion, defecation, egg-laying, and associative learning behaviors in N2 C. elegans, while no significant alterations were observed in the VC1241 C. elegans. Furthermore, we conducted a 4D label-free proteomics analysis and screened 504 key proteins significantly affected by HgS nanoparticles through Skp1. These proteins play pivotal roles in various pathways, including SNARE interactions in vesicular transport, TGF-beta signaling pathway, calcium signaling pathway, FoxO signaling pathway, etc. In summary, HgS nanoparticles at high doses suppress the neurobehavioral functions of C. elegans through a Skp1-dependent mechanism.
      Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
      (Copyright © 2024 Elsevier Ltd. All rights reserved.)
    • Contributed Indexing:
      Keywords: Caenorhabditis elegans; Cinnabar; Mercuric sulfide; Nanoparticles; Neurobehavior; Skp1
    • Accession Number:
      ZI0T668SF1 (cinnabar)
      0 (S-Phase Kinase-Associated Proteins)
      0 (Mercury Compounds)
      0 (Caenorhabditis elegans Proteins)
    • Publication Date:
      Date Created: 20240308 Date Completed: 20240329 Latest Revision: 20240329
    • Publication Date:
      20240329
    • Accession Number:
      10.1016/j.fct.2024.114576
    • Accession Number:
      38458533