Tektin bundle interacting protein, TEKTIP1, functions to stabilize the tektin bundle and axoneme in mouse sperm flagella.

Publisher: Springer Country of Publication: Switzerland NLM ID: 9705402 Publication Model: Electronic Cited Medium: Internet ISSN: 1420-9071 (Electronic) Linking ISSN: 1420682X NLM ISO Abbreviation: Cell Mol Life Sci Subsets: MEDLINE

Publication: Basel : Springer
Original Publication: Basel ; Boston : Birkhauser, c1997-

Axoneme* ; Semen* ; Microtubule Proteins*; Humans ; Male ; Animals ; Mice ; Female ; Cryoelectron Microscopy ; Sperm Motility ; Spermatozoa ; Flagella

Tektins are microtubule inner proteins (MIPs) and localize at the inside lumen of doublet microtubules (DMTs) of cilia/flagella. TEKTIP1, a newly identified protein by cryo-electron microscopy (cryo-EM), is proposed to be localized at the center of the tektin bundle and hypothesized to recruit tektins or stabilize the bundle. However, the physiological role of TEKTIP1 is unknown. In this study, we generated Tektip1-knockout (Tektip1 ^-/- ) mice and showed that they were male subfertile primarily due to reduced sperm motility. A high percentage of sperm from Tektip1 ^-/- mice showed moderately disorganized axoneme structures and abnormal flagellar waveforms. TEKTIP1 predominately interacted with TEKT3 among tektins. Loss of TEKTIP1 partially disturbed the organization of tektin bundle by mainly affecting the native status of TEKT3 and its interaction with other tektins. Collectively, our study reveals the physiological role and potential molecular mechanism of TEKTIP1 in axonemal structure and sperm motility, highlights the importance of MIPs in stabilizing DMTs, and suggests a potential relevance of TEKTIP1 deficiency to human asthenospermia. Tektip1 ^-/-  mice will be an excellent animal model to study the DMT organization of sperm flagella using cryo-EM in future.
(© 2024. The Author(s).)

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32370905 National Natural Science Foundation of China; 2019YFA0802101 National Key Research and Development Project

Keywords: Knockout mice; Microtubule inner protein; Sperm motility; TEKTIP1; Tektins

0 (tektins)
0 (Microtubule Proteins)

Date Created: 20240306 Date Completed: 20240308 Latest Revision: 20240309

20240309

PMC10917850

10.1007/s00018-023-05081-3

38448737