Molecular characterization and toxigenic profiles of Bacillus cereus isolates from foodstuff and food poisoning outbreaks in Brazil.

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  • Additional Information
    • Source:
      Publisher: Springer International Publishing Country of Publication: Brazil NLM ID: 101095924 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1678-4405 (Electronic) Linking ISSN: 15178382 NLM ISO Abbreviation: Braz J Microbiol Subsets: MEDLINE
    • Publication Information:
      Publication: 2019- : Switzerland, AG : Springer International Publishing
      Original Publication: Rio de Janeiro, RJ, Brasil : Sociedade Brasileira de Microbiologia
    • Subject Terms:
      Bacillus cereus*/genetics ; Bacillus cereus*/isolation & purification ; Bacillus cereus*/classification ; Bacillus cereus*/pathogenicity ; Foodborne Diseases*/microbiology ; Foodborne Diseases*/epidemiology ; Disease Outbreaks* ; Enterotoxins*/genetics ; Food Microbiology*; Brazil/epidemiology ; Humans ; Virulence Factors/genetics ; Gram-Positive Bacterial Infections/microbiology ; Gram-Positive Bacterial Infections/epidemiology
    • Abstract:
      Bacillus cereus sensu stricto (s.s.) is a well-known foodborne pathogen that produces a range of enterotoxins and is able to cause two different types of foodborne illnesses-the emetic and the diarrheal syndromes. In this study, 54 B. cereus s.s. strains isolated from foodstuff and foods involved in food poisoning outbreaks were characterized according to the presence of toxin-encoding genes, virulence-encoding genes, and panC typing. Most isolates were assigned to panC groups IV (61.1%) and III (25.9%), but members of groups II and V could also be found. Investigation of specific alleles revealed high numbers of isolates carrying toxin and other virulence genes including nheA (100%), nheB (100%), hblA (79.6%), hblC (79.6%), hblD (74.1%), cytK-2 (61.1%), clo (100%), pc-plc (75.9%), sph (68.5%), pi-plc (66.6%), hlyIII (62.9%), and hlyII (24.1%). All isolates were negative for ces and cytK-1. In summary, we detected various enterotoxin and other virulence factor genes associated with diarrheal syndrome in strains analyzed, implicated or not with food poisoning. Furthermore, the most isolates analyzed belong to high-risk phylogenetic groups' panC types III and IV. Our study provides a convenient molecular scheme for characterization of B. cereus s.s. strains responsible for food poisoning outbreaks in order to improve the monitoring and investigation and assess emerging clusters and diversity of strains.
      (© 2024. The Author(s) under exclusive licence to Sociedade Brasileira de Microbiologia.)
    • References:
      Euro Surveill. 2016 Dec 1;21(48):. (PMID: 27934583)
      J Food Prot. 2017 Jul;80(7):1060-1065. (PMID: 28556679)
      Food Microbiol. 2010 May;27(3):347-55. (PMID: 20227599)
      Appl Environ Microbiol. 2018 Mar 1;84(6):. (PMID: 29330180)
      J Infect Dev Ctries. 2014 Mar 13;8(3):254-7. (PMID: 24619253)
      Crit Rev Microbiol. 2011 Aug;37(3):188-213. (PMID: 21417966)
      J Food Prot. 2005 Oct;68(10):2123-30. (PMID: 16245717)
      Foodborne Pathog Dis. 2012 Dec;9(12):1130-6. (PMID: 23237409)
      J Clin Microbiol. 2010 Sep;48(9):3388-91. (PMID: 20660215)
      BMC Genomics. 2019 May 29;20(1):436. (PMID: 31142281)
      Int J Food Microbiol. 2011 Dec 15;151(3):277-83. (PMID: 21978657)
      Int J Food Microbiol. 2009 Sep 30;135(1):15-21. (PMID: 19665814)
      FEMS Microbiol Lett. 2018 Feb 1;365(3):. (PMID: 29390131)
      Environ Microbiol. 2008 Apr;10(4):851-65. (PMID: 18036180)
      Microbiology (Reading). 2000 Dec;146 Pt 12:3033-3039. (PMID: 11101661)
      Antonie Van Leeuwenhoek. 2006 Feb;89(2):239-49. (PMID: 16710635)
      Food Microbiol. 2020 Feb;85:103279. (PMID: 31500702)
      Front Microbiol. 2019 Feb 12;10:144. (PMID: 30809204)
      BMC Microbiol. 2019 Aug 24;19(1):196. (PMID: 31445510)
      Int J Food Microbiol. 2018 Dec 2;286:80-89. (PMID: 30053697)
      Food Microbiol. 2011 Apr;28(2):199-208. (PMID: 21315974)
      Food Microbiol. 2019 Dec;84:103276. (PMID: 31421762)
      Int J Syst Evol Microbiol. 2013 Jan;63(Pt 1):31-40. (PMID: 22328607)
      FEMS Microbiol Ecol. 2013 Jun;84(3):433-50. (PMID: 23488744)
      FEMS Microbiol Lett. 2006 Jun;259(1):74-80. (PMID: 16684105)
      J Food Prot. 2008 Oct;71(10):2115-8. (PMID: 18939763)
      Front Microbiol. 2018 Aug 23;9:1915. (PMID: 30190709)
      Front Microbiol. 2017 Apr 12;8:627. (PMID: 28446903)
      Infect Immun. 2003 Nov;71(11):6591-606. (PMID: 14573681)
      Int J Food Microbiol. 2016 Sep 2;232:80-6. (PMID: 27257745)
      Int J Food Microbiol. 2011 May 14;147(1):12-6. (PMID: 21440319)
      Int J Food Microbiol. 2018 Oct 20;283:59-64. (PMID: 30099996)
      BMC Microbiol. 2006 Mar 02;6:20. (PMID: 16512902)
      Mol Cell Proteomics. 2010 Jul;9(7):1486-98. (PMID: 20368289)
    • Grant Information:
      process HBM-00007-17 FAPEMIG
    • Contributed Indexing:
      Keywords: Bacillus cereus sensu stricto (s.s.); panC group; Food poisoning; Toxin-encoding genes; Virulence-encoding genes
    • Accession Number:
      0 (Enterotoxins)
      0 (Virulence Factors)
    • Publication Date:
      Date Created: 20240306 Date Completed: 20240605 Latest Revision: 20240608
    • Publication Date:
      20240608
    • Accession Number:
      PMC11153380
    • Accession Number:
      10.1007/s42770-024-01283-4
    • Accession Number:
      38446406