Erythropoietin effects on cryopreserved/transplanted cat ovarian tissue: A comparison of two incubation methods.

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  • Additional Information
    • Source:
      Publisher: Elsevier Country of Publication: Netherlands NLM ID: 0006252 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1090-2392 (Electronic) Linking ISSN: 00112240 NLM ISO Abbreviation: Cryobiology Subsets: MEDLINE
    • Publication Information:
      Publication: <2000- > : Amsterdam : Elsevier
      Original Publication: San Diego, CA : Academic Press
    • Subject Terms:
      Cryopreservation*/methods ; Cryopreservation*/veterinary ; Erythropoietin*/pharmacology ; Ovary*/drug effects ; Ovary*/transplantation ; Mice, Nude* ; Transplantation, Heterologous*; Animals ; Female ; Cats ; Mice ; Ovarian Follicle/drug effects ; Cryoprotective Agents/pharmacology ; Neovascularization, Physiologic/drug effects
    • Abstract:
      Many feline species are currently threatened with extinction. Therefore, germplasm bank establishment has become imperative. However, cryoinjury and ischemia-reperfusion injury pose significant obstacles to both cryopreservation and xenotransplantation. In this regard, erythropoietin (Epo) represents a potential alternative strategy due to its properties. This study aimed to assess the incubation of domestic cat ovarian tissue in Epo, both before and after cryopreservation, and investigate its effectiveness in promoting revascularization following xenotransplantation. Sixteen ovaries from 8 healthy cats were sliced following elective bilateral ovariohysterectomy (OHE). Subsequently, 8 fragments measuring 3 mm³ each were obtained from the cortical region of each ovary. The fragments were allocated into 3 treatment groups: Cryo group, fragments were cryopreserved, thawed and immediately transplanted; Cryo + Epo group, fragments were first cryopreserved in nitrogen, thawed, incubated in Epo (100 IU) for 2h and transplanted; and the Epo + Cryo group, in which fragments were first incubated in Epo (100 IU) for 2h, cryopreserved, thawed and immediately transplanted. The fragments were then xenotransplanted into the dorsal subcutaneous region of ovariectomized female nude mice and retrieved at 7, 14, 21, and 28 days post-transplantation. The results indicated that Epo effectively enhanced follicular survival, preservation of viability, and tissue revascularization. The Epo + Cryo group displayed better revascularization rates on D14 and D21 post-transplantation and an increase in primordial and growing follicles on D28, the Cryo + Epo group exhibited significantly more follicles on D14 and D21, with fewer degenerated follicles.
      Competing Interests: Declaration of competing interest The authors declare no conflict of interests.
      (Copyright © 2024 Society for Cryobiology. Published by Elsevier Inc. All rights reserved.)
    • Contributed Indexing:
      Keywords: Angiogenesis; Cryopreservation; Germplasm bank; Ischemia-reperfusion; Ovarian follicles; Ovarian tissue grafting
    • Accession Number:
      11096-26-7 (Erythropoietin)
      0 (Cryoprotective Agents)
    • Publication Date:
      Date Created: 20240229 Date Completed: 20240609 Latest Revision: 20240609
    • Publication Date:
      20250114
    • Accession Number:
      10.1016/j.cryobiol.2024.104861
    • Accession Number:
      38423494