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Developmental validation of the STRSeqTyper122 kit for massively parallel sequencing of forensic STRs.
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- Author(s): Guo LL;Guo LL; Yuan JH; Yuan JH; Zhang C; Zhang C; Zhao J; Zhao J; Yao YR; Yao YR; Guo KL; Guo KL; Meng Y; Meng Y; Ji AQ; Ji AQ; Kang KL; Kang KL; Wang L; Wang L; Wang L
- Source:
International journal of legal medicine [Int J Legal Med] 2024 Jul; Vol. 138 (4), pp. 1255-1264. Date of Electronic Publication: 2024 Feb 28.- Publication Type:
Journal Article; Validation Study- Language:
English - Source:
- Additional Information
- Source: Publisher: Springer International Country of Publication: Germany NLM ID: 9101456 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1437-1596 (Electronic) Linking ISSN: 09379827 NLM ISO Abbreviation: Int J Legal Med Subsets: MEDLINE
- Publication Information: Original Publication: Heidelberg, FRG : Springer International, c1990-
- Subject Terms: Microsatellite Repeats* ; High-Throughput Nucleotide Sequencing* ; DNA Fingerprinting*/methods; Humans ; Amelogenin/genetics ; Reproducibility of Results ; Sequence Analysis, DNA/methods ; Genotype ; Polymerase Chain Reaction ; Species Specificity ; Male ; Animals ; DNA Degradation, Necrotic ; Electrophoresis, Capillary ; Female
- Abstract: Massively parallel sequencing allows for integrated genotyping of different types of forensic markers, which reduces DNA consumption, simplifies experimental processes, and provides additional sequence-based genetic information. The STRseqTyper122 kit genotypes 63 autosomal STRs, 16 X-STRs, 42 Y-STRs, and the Amelogenin locus. Amplicon sizes of 117 loci were below 300 bp. In this study, MiSeq FGx sequencing metrics for STRseqTyper122 were presented. The genotyping accuracy of this kit was examined by comparing to certified genotypes of NIST standard reference materials and results from five capillary electrophoresis-based kits. The sensitivity of STRseqTyper122 reached 125 pg, and > 80% of the loci were correctly called with 62.5 pg and 31.25 pg input genomic DNA. Repeatability, species specificity, and tolerance for DNA degradation and PCR inhibitors of this kit were also evaluated. STRseqTyper122 demonstrated reliable performance with routine case-work samples and provided a powerful tool for forensic applications.
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- Contributed Indexing: Keywords: Developmental validation; Forensic DNA; Massively parallel sequencing; Short tandem repeat
- Accession Number: 0 (Amelogenin)
- Publication Date: Date Created: 20240228 Date Completed: 20240610 Latest Revision: 20240610
- Publication Date: 20240611
- Accession Number: 10.1007/s00414-024-03195-2
- Accession Number: 38416217
- Source:
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