Developmental validation of the STRSeqTyper122 kit for massively parallel sequencing of forensic STRs.

Publisher: Springer International Country of Publication: Germany NLM ID: 9101456 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1437-1596 (Electronic) Linking ISSN: 09379827 NLM ISO Abbreviation: Int J Legal Med Subsets: MEDLINE

Original Publication: Heidelberg, FRG : Springer International, c1990-

Microsatellite Repeats* ; High-Throughput Nucleotide Sequencing* ; DNA Fingerprinting*/methods; Humans ; Amelogenin/genetics ; Reproducibility of Results ; Sequence Analysis, DNA/methods ; Genotype ; Polymerase Chain Reaction ; Species Specificity ; Male ; Animals ; DNA Degradation, Necrotic ; Electrophoresis, Capillary ; Female

Massively parallel sequencing allows for integrated genotyping of different types of forensic markers, which reduces DNA consumption, simplifies experimental processes, and provides additional sequence-based genetic information. The STRseqTyper122 kit genotypes 63 autosomal STRs, 16 X-STRs, 42 Y-STRs, and the Amelogenin locus. Amplicon sizes of 117 loci were below 300 bp. In this study, MiSeq FGx sequencing metrics for STRseqTyper122 were presented. The genotyping accuracy of this kit was examined by comparing to certified genotypes of NIST standard reference materials and results from five capillary electrophoresis-based kits. The sensitivity of STRseqTyper122 reached 125 pg, and > 80% of the loci were correctly called with 62.5 pg and 31.25 pg input genomic DNA. Repeatability, species specificity, and tolerance for DNA degradation and PCR inhibitors of this kit were also evaluated. STRseqTyper122 demonstrated reliable performance with routine case-work samples and provided a powerful tool for forensic applications.
(© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)

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81601649 National Natural Science Foundation of China; 2017YFC0803503 Key Technologies Research and Development Program; 2019JB045 Institute of Forensic Science, Ministry of Public Security

Keywords: Developmental validation; Forensic DNA; Massively parallel sequencing; Short tandem repeat

0 (Amelogenin)

Date Created: 20240228 Date Completed: 20240610 Latest Revision: 20240610

20240611

10.1007/s00414-024-03195-2

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