Enhanced capacity of a leaf beetle to combat dual stress from entomopathogens and herbicides mediated by associated microbiota.

Publisher: Wiley Publishing Asia Pty Ltd Country of Publication: Australia NLM ID: 101492420 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1749-4877 (Electronic) Linking ISSN: 17494869 NLM ISO Abbreviation: Integr Zool Subsets: MEDLINE

Publication: 2012-: Richmond, Vic., Australia : Wiley Publishing Asia Pty Ltd
Original Publication: 2006-2012: [Oxford, England] : Blackwell Publishing

Herbicides*/toxicity ; Herbicides*/pharmacology ; Glyphosate* ; Coleoptera*/microbiology ; Coleoptera*/drug effects ; Glycine*/analogs & derivatives ; Glycine*/pharmacology ; Microbiota*/drug effects; Animals ; Aspergillus/drug effects ; Stress, Physiological ; Bacteria/drug effects ; Pseudomonas/drug effects

Herbicides have demonstrated their impact on insect fitness by affecting their associated microbiota or altering the virulence of entomopathogenic fungi toward insects. However, limited research has explored the implications of herbicide stress on the intricate tripartite interaction among insects, associated bacterial communities, and entomopathogens. In this study, we initially demonstrated that associated bacteria confer a leaf beetle, Plagiodera versicolora, with the capability to resist the entomopathogenic fungus Aspergillus nomius infection, a capability sustained even under herbicide glyphosate stress. Further analysis of the associated microbiota revealed a significant alteration in abundance and composition due to glyphosate treatment. The dominant bacterium, post A. nomius infection or following a combination of glyphosate treatments, exhibited strong suppressive effects on fungal growth. Additionally, glyphosate markedly inhibited the pathogenic associated bacterium Pseudomonas though it inhibited P. versicolora's immunity, ultimately enhancing the beetle's tolerance to A. nomius. In summary, our findings suggest that the leaf beetle's associated microbiota bestow an augmented resilience against the dual stressors of both the entomopathogen and glyphosate. These results provide insight into the effects of herbicide residues on interactions among insects, associated bacteria, and entomopathogenic fungi, holding significant implications for pest control and ecosystem assessment.
(© 2024 International Society of Zoological Sciences, Institute of Zoology/Chinese Academy of Sciences and John Wiley & Sons Australia, Ltd.)

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Keywords: associated microbiota; entomopathogenic fungi; herbicides; leaf beetle

0 (Herbicides)
4632WW1X5A (Glyphosate)
TE7660XO1C (Glycine)

Date Created: 20240221 Date Completed: 20241104 Latest Revision: 20241104

20241105

10.1111/1749-4877.12812

38379126