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Determinants of viscoelasticity and flow activation energy in biomolecular condensates.
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- Additional Information
- Source:
Publisher: American Association for the Advancement of Science Country of Publication: United States NLM ID: 101653440 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2375-2548 (Electronic) Linking ISSN: 23752548 NLM ISO Abbreviation: Sci Adv Subsets: MEDLINE
- Publication Information:
Original Publication: Washington, DC : American Association for the Advancement of Science, [2015]-
- Subject Terms:
- Abstract:
The form and function of biomolecular condensates are intimately linked to their material properties. Here, we integrate microrheology with molecular simulations to dissect the physical determinants of condensate fluid phase dynamics. By quantifying the timescales and energetics of network relaxation in a series of heterotypic viscoelastic condensates, we uncover distinctive roles of sticker motifs, binding energy, and chain length in dictating condensate dynamical properties. We find that the mechanical relaxation times of condensate-spanning networks are determined by both intermolecular interactions and chain length. We demonstrate, however, that the energy barrier for network reconfiguration, termed flow activation energy, is independent of chain length and only varies with the strengths of intermolecular interactions. Biomolecular diffusion in the dense phase depends on a complex interplay between viscoelasticity and flow activation energy. Our results illuminate distinctive roles of chain length and sequence-specific multivalent interactions underlying the complex material and transport properties of biomolecular condensates.
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- Grant Information:
R35 GM138186 United States GM NIGMS NIH HHS; R35 GM138243 United States GM NIGMS NIH HHS
- Publication Date:
Date Created: 20240216 Date Completed: 20240219 Latest Revision: 20240225
- Publication Date:
20240225
- Accession Number:
PMC10871536
- Accession Number:
10.1126/sciadv.adi6539
- Accession Number:
38363841
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