Quantitative Analysis of the Human Semen Phosphorometabolome by 31 P-NMR.

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  • Additional Information
    • Source:
      Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE
    • Publication Information:
      Original Publication: Basel, Switzerland : MDPI, [2000-
    • Subject Terms:
      Semen*/metabolism ; Asthenozoospermia*/metabolism ; Organophosphates*; Humans ; Male ; Phosphorylcholine/metabolism ; Sperm Motility ; Spermatozoa/metabolism ; Phosphorus/metabolism ; Semen Analysis
    • Abstract:
      Phosphorus-containing metabolites occupy a prominent position in cell pathways. The phosphorometabolomic approach in human sperm samples will deliver valuable information as new male fertility biomarkers could emerge. This study analyzed, by 31 P-NMR, seminal plasma and whole semen from asthenozoospermic and normozoospermic samples (71% vs. 27% and 45% vs. 17%, total and progressive sperm motility, respectively), and also ejaculates from healthy donors. At least 16 phosphorus-containing metabolites involved in central energy metabolism and phospholipid, nucleotide, and nicotinamide metabolic pathways were assigned and different abundances between the samples with distinct sperm quality was detected. Specifically, higher levels of phosphocholine, glucose-1-phosphate, and to a lesser degree, acetyl phosphate were found in the asthenozoospermic seminal plasma. Notably, the phosphorometabolites implicated in lipid metabolism were highlighted in the seminal plasma, while those associated with carbohydrate metabolism were more abundant in the spermatozoa. Higher levels of phosphocholine, glucose-1-phosphate, and acetyl phosphate in the seminal plasma with poor quality suggest their crucial role in supporting sperm motility through energy metabolic pathways. In the seminal plasma, phosphorometabolites related to lipid metabolism were prominent; however, spermatozoa metabolism is more dependent on carbohydrate-related energy pathways. Understanding the presence and function of sperm phosphorylated metabolites will enhance our knowledge of the metabolic profile of healthy human sperm, improving assessment and differential diagnosis.
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    • Grant Information:
      IB20154 Government of Extremadura; GR18094 Government of Extremadura
    • Contributed Indexing:
      Keywords: NMR-based phosphoromics; asthenozoospermia; human sperm; metabolomics; seminal plasma
    • Accession Number:
      590-54-5 (acetyl phosphate)
      107-73-3 (Phosphorylcholine)
      27YLU75U4W (Phosphorus)
      0 (Organophosphates)
    • Publication Date:
      Date Created: 20240210 Date Completed: 20240214 Latest Revision: 20240214
    • Publication Date:
      20240214
    • Accession Number:
      PMC10855173
    • Accession Number:
      10.3390/ijms25031682
    • Accession Number:
      38338962