Mapping the microcarrier design pathway to modernise clinical mesenchymal stromal cell expansion.

Publisher: Elsevier Science Publishers Country of Publication: England NLM ID: 8310903 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-3096 (Electronic) Linking ISSN: 01677799 NLM ISO Abbreviation: Trends Biotechnol Subsets: MEDLINE

Publication: Barking : Elsevier Science Publishers
Original Publication: [Amsterdam, Netherlands : Elsevier Science Publishers B.V. (Biomedical Division), c1983-

Mesenchymal Stem Cells*/cytology ; Cell Culture Techniques*/methods ; Cell Culture Techniques*/instrumentation; Humans ; Cell Proliferation

Microcarrier expansion systems show exciting potential to revolutionise mesenchymal stromal cell (MSC)-based clinical therapies by providing an opportunity for economical large-scale expansion of donor- and patient-derived cells. The poor reproducibility and efficiency of cell expansion on commercial polystyrene microcarriers have driven the development of novel microcarriers with tuneable physical, mechanical, and cell-instructive properties. These new microcarriers show innovation toward improving cell expansion outcomes, although their limited biological characterisation and compatibility with dynamic culture systems suggest the need to realign the microcarrier design pathway. Clear headway has been made toward developing infrastructure necessary for scaling up these technologies; however, key challenges remain in characterising the wholistic effects of microcarrier properties on the biological fate and function of expanded MSCs.
Competing Interests: Declaration of interests No interests are declared.
(Copyright © 2024 Elsevier Ltd. All rights reserved.)

Keywords: biomaterials; bioreactors; cell attachment; differentiation; dynamic culture; surface modifications

Date Created: 20240206 Date Completed: 20240704 Latest Revision: 20240705

20240706

10.1016/j.tibtech.2024.01.001

38320911