Sample preparation for fatty acid analysis in biological samples with mass spectrometry-based strategies.

Publisher: Springer-Verlag Country of Publication: Germany NLM ID: 101134327 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1618-2650 (Electronic) Linking ISSN: 16182642 NLM ISO Abbreviation: Anal Bioanal Chem Subsets: MEDLINE

Original Publication: Heidelberg : Springer-Verlag, 2002-

Fatty Acids*/analysis ; Fatty Acids, Unsaturated*; Mass Spectrometry/methods ; Gas Chromatography-Mass Spectrometry/methods ; Liquid Chromatography-Mass Spectrometry

Fatty acids (FAs) have attracted many interests for their pivotal roles in many biological processes. Imbalance of FAs is related to a variety of diseases, which makes the measurement of them important in biological samples. Over the past two decades, mass spectrometry (MS) has become an indispensable technique for the analysis of FAs owing to its high sensitivity and precision. Due to complex matrix effect of biological samples and inherent poor ionization efficiency of FAs in MS, sample preparation including extraction and chemical derivatization prior to analysis are often employed. Here, we describe an updated overview of FA extraction techniques, as well as representative derivatization methods utilized in different MS platforms including gas chromatography-MS, liquid chromatography-MS, and mass spectrometry imaging based on different chain lengths of FAs. Derivatization strategies for the identification of double bond location in unsaturated FAs are also summarized and highlighted. The advantages, disadvantages, and prospects of these methods are compared and discussed. This review provides the development and valuable information for sample pretreatment approaches and qualitative and quantitative analysis of interested FAs using different MS-based platforms in complex biological matrices. Finally, the challenges of FA analysis are summarized and the future perspectives are prospected.
(© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH, DE part of Springer Nature.)

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202303021211109 Natural Science Foundation of Shanxi Province; 22125401 National Natural Science Foundation of China; 81973287 National Natural Science Foundation of China

Keywords: Derivatization; Extraction; Fatty acids; Mass spectrometry

0 (Fatty Acids)
0 (Fatty Acids, Unsaturated)

Date Created: 20240206 Date Completed: 20240321 Latest Revision: 20240711

20240711

10.1007/s00216-024-05185-0

38319358