In vitro fermentation characteristics of different types of fiber-rich ingredients by pig fecal inoculum.

Publisher: John Wiley & Sons Country of Publication: England NLM ID: 0376334 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1097-0010 (Electronic) Linking ISSN: 00225142 NLM ISO Abbreviation: J Sci Food Agric Subsets: MEDLINE

Publication: <2005-> : Chichester, West Sussex : John Wiley & Sons
Original Publication: London, Society of Chemical Industry.

Fermentation* ; Dietary Fiber*/metabolism ; Dietary Fiber*/analysis ; Feces*/microbiology ; Bacteria*/metabolism ; Bacteria*/classification ; Bacteria*/genetics ; Bacteria*/isolation & purification ; Fatty Acids, Volatile*/metabolism ; Animal Feed*/analysis ; Gastrointestinal Microbiome*; Animals ; Swine ; Zea mays/chemistry ; Zea mays/metabolism ; Beta vulgaris/chemistry ; Beta vulgaris/metabolism ; Beta vulgaris/microbiology ; Medicago sativa/chemistry ; Medicago sativa/metabolism ; Medicago sativa/microbiology ; Oryza/metabolism ; Oryza/chemistry ; Oryza/microbiology

Background: Dietary fibers with varying physicochemical properties have different fermentation characteristics, which may differently impact host health. The present study aimed to determine the fermentation characteristics including gas production kinetics, short-chain fatty acids (SCFAs) production and microbial composition of different fibrous ingredients using in vitro fermentation by fecal microbiota.
Results: Sugar beet pule (SBP), wheat bran (WB), dried corn distillers grains with solubles (DDGS), rice bran (RB) and alfalfa meal (AM) were selected to fermentation in vitro for 36 h. The results showed that SBP had the greatest gas production. SBP had the highest in vitro dry matter fermentability (IVDMF) and production of acetate, propionate and total SCFAs, followed by WB, which were all greater than DDGS, AM and RB. The alpha-diversity was higher in the DDGS, AM and RB groups than in the WB and SBP groups. Differences in microbial community composition were observed among groups. The relative abundance of Treponema was highest in WB group. RB group showed lower Prevotella abundance than other groups but had higher Succinivibrio abundance. Interestingly, the Lactobacillus reached the highest abundances in the DDGS group. Correlation analysis indicated that the relative abundance of Treponema and Prevotella was positively associated with the gas production, IVDMF and SCFAs, whereas norank_f_Muribaculaceae, Rikenellaceae_RC9_gut_group, Lysinibacillus and Succinivibrio were the opposite.
Conclusion: Collectively, WB and SBP were fermented rapidly by fecal microbiota compared to DDGS, AM and RB. Different fiber sources have different fiber compositions and fermentation properties that affect the microbial compositins and SCFAs production. © 2024 Society of Chemical Industry.
(© 2024 Society of Chemical Industry.)

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2022YFD1300605 the National Key R&D Program of China; 2022YFD1300600 the National Key R&D Program of China; 2022TSGC2029 Shandong Province Innovation Capability Improvement Project for Small and Medium-sized Scientific and Technological Enterprises; 202228037 Jinan Introductory Innovation Team Project

Keywords: SCFAs; fibers; gas production; in vitro fermentation; microbiota

0 (Dietary Fiber)
0 (Fatty Acids, Volatile)

Date Created: 20240203 Date Completed: 20240614 Latest Revision: 20240614

20240614

10.1002/jsfa.13355

38308576