Edwardsiella piscicida causes iron storage disorders by an autophagy pathway in fish monocytes/macrophages.

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  • Additional Information
    • Source:
      Publisher: Academic Press Country of Publication: England NLM ID: 9505220 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1095-9947 (Electronic) Linking ISSN: 10504648 NLM ISO Abbreviation: Fish Shellfish Immunol Subsets: MEDLINE
    • Publication Information:
      Original Publication: London ; New York : Academic Press, c1991-
    • Subject Terms:
      Hemochromatosis* ; Edwardsiella*/physiology ; Fish Diseases*/microbiology ; Enterobacteriaceae Infections*/veterinary ; Enterobacteriaceae Infections*/microbiology; Animals ; Monocytes/metabolism ; Fishes/metabolism ; Macrophages/metabolism ; Autophagy ; Iron/metabolism ; Ferritins/genetics ; Bacterial Proteins/metabolism
    • Abstract:
      Edwardsiella piscicida (E. piscicida) is a gram-negative pathogen that survives in intracellular environment. Currently, the interplay between E. piscicida and host cells has not been completely explored. In this study, we found that E. piscicida disturbed iron homeostasis in grass carp monocytes/macrophages to maintain its own growth. Further investigation revealed the bacteria induced an increase of intracellular iron, which was subjected to the degradation of ferritin. Moreover, the autophagy inhibitor impeded the degradation of ferritin and increase of intracellular iron in E. piscicida-infected monocytes/macrophages, implying possible involvement of autophagy response in the process of E. piscicida-broken iron homeostasis. Along this line, confocal microscopy observed that E. piscicida elicited the colocalization of ferritin with LC3-positive autophagosome in the monocytes/macrophages, indicating that E. piscicida mediated the degradation of ferritin possibly through the autophagic pathway. These results deepened our understanding of the interaction between E. piscicida and fish cells, hinting that the disruption of iron homeostasis was an important factor for pathogenicity of E. piscicida. They also indicated that autophagy was a possible mechanism governing intracellular iron metabolism in response to E. piscicida infection and might offer a new avenue for anti-E. piscicida strategies in the future.
      (Copyright © 2024 Elsevier Ltd. All rights reserved.)
    • Contributed Indexing:
      Keywords: Autophagy; Edwardsiella piscicida; Ferritin; Grass carp; Iron homeostasis
    • Accession Number:
      E1UOL152H7 (Iron)
      9007-73-2 (Ferritins)
      0 (Bacterial Proteins)
    • Subject Terms:
      Edwardsiella piscicida
    • Publication Date:
      Date Created: 20240201 Date Completed: 20240219 Latest Revision: 20240219
    • Publication Date:
      20240219
    • Accession Number:
      10.1016/j.fsi.2024.109417
    • Accession Number:
      38301814