Does YAP influence cell proliferation and apoptosis in benign epithelial odontogenic lesions?

Publisher: Munksgaard Country of Publication: Denmark NLM ID: 9508565 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1601-0825 (Electronic) Linking ISSN: 1354523X NLM ISO Abbreviation: Oral Dis Subsets: MEDLINE

Publication: 2001- : Copenhagen, Denmark : Munksgaard
Original Publication: Houndmills, Basingstoke, Hampshire, UK : Stockton Press, c1995-

Ameloblastoma*/pathology ; Ameloblastoma*/metabolism ; Odontogenic Cysts*/pathology ; Odontogenic Cysts*/metabolism ; Cell Proliferation* ; Apoptosis* ; Dentigerous Cyst*/pathology ; Dentigerous Cyst*/metabolism ; Ki-67 Antigen*/metabolism ; Ki-67 Antigen*/analysis ; YAP-Signaling Proteins* ; Cyclin D1*/metabolism ; Adaptor Proteins, Signal Transducing*/metabolism ; Proto-Oncogene Proteins c-bcl-2*/analysis ; Proto-Oncogene Proteins c-bcl-2*/metabolism; Humans ; Transcription Factors/analysis ; Dental Sac/pathology ; Dental Sac/metabolism ; Immunohistochemistry ; Odontogenic Tumors/pathology ; Odontogenic Tumors/metabolism ; Epithelial Cells/pathology ; Epithelial Cells/metabolism

Objective: To analyze the immunohistochemical expression of YAP and its correlation with markers involved in cell proliferation and apoptosis in benign epithelial odontogenic lesions.
Study Design: The sample consisted of 95 cases of odontogenic lesions (25 dentigerous cysts, 30 non-syndromic odontogenic keratocysts, 30 conventional ameloblastomas, and 10 unicystic ameloblastomas) and 10 dental follicles used as normal odontogenic tissue. The histological sections were submitted to immunohistochemistry with YAP, cyclin D1, Ki-67, and Bcl-2 antibodies. Immunoexpression was analyzed qualitatively and quantitatively using an adapted method. The collected data were analyzed descriptively and statistically (p ≤ 0.05).
Results: The highest YAP expression was observed in odontogenic keratocysts, followed by unicystic ameloblastomas and conventional ameloblastomas, which exhibited moderate immunoreactivity predominantly in peripheral cells. Furthermore, significant differences in YAP immunoexpression were observed between the groups analyzed, with significant positive correlations between YAP and cyclin D1 in dentigerous cysts and unicystic ameloblastomas and between YAP and Ki-67 in unicystic ameloblastomas (p < 0.05). However, there were no statistically significant correlations between YAP and Bcl-2 immunoexpression in the groups studied.
Conclusion: YAP may influence epithelial cell proliferation in odontogenic cysts and tumors, suggesting its possible participation in the progression of the odontogenic lesions studied.
(© 2024 Wiley Periodicals LLC.)

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Keywords: apoptosis; cell proliferation; immunohistochemistry; odontogenic cysts; odontogenic tumors; yes‐associated protein

0 (Ki-67 Antigen)
0 (YAP-Signaling Proteins)
0 (YAP1 protein, human)
136601-57-5 (Cyclin D1)
0 (Adaptor Proteins, Signal Transducing)
0 (Proto-Oncogene Proteins c-bcl-2)
0 (Transcription Factors)
0 (CCND1 protein, human)
0 (BCL2 protein, human)

Date Created: 20240201 Date Completed: 20241007 Latest Revision: 20241008

20241009

10.1111/odi.14882

38297810