Nestin-expressing cells are mitotically active in the mammalian inner ear.

Publisher: Elsevier/North-Holland Biomedical Press Country of Publication: Netherlands NLM ID: 7900445 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1878-5891 (Electronic) Linking ISSN: 03785955 NLM ISO Abbreviation: Hear Res Subsets: MEDLINE

Original Publication: Amsterdam, Elsevier/North-Holland Biomedical Press.

Cochlea*/metabolism ; Vestibule, Labyrinth*/metabolism; Animals ; Mice ; Hair Cells, Auditory/metabolism ; Nestin/genetics ; Nestin/metabolism ; Saccule and Utricle/metabolism

Nestin expression is associated with pluripotency. Growing evidence suggests nestin is involved in hair cell development. The objective of this study was to investigate the morphology and role of nestin-expressing cells residing in the early postnatal murine inner ear. A lineage-tracing nestin reporter mouse line was used to further characterize these cells. Their cochleae and vestibular organs were immunostained and whole-mounted for cell counting. We found Nestin-expressing cells present in low numbers throughout the inner ear. Three morphotypes were observed: bipolar, unipolar, and globular. Mitotic activity was noted in nestin-expressing cells in the cochlea, utricle, saccule, and crista. Nestin-expressing cell characteristics were then observed after hair cell ablation in two mouse models. First, a reporter model demonstrated nestin expression in a significantly higher proportion of hair cells after hair cell ablation than in control cochleae. However, in a lineage tracing nestin reporter mouse, none of the new hair cells which repopulated the organ of Corti after hair cell ablation expressed nestin, nor did the nestin-expressing cells change in morphotype. In conclusion, Nestin-expressing cells were identified in the cochlea and vestibular organs. After hair cell ablation, nestin-expressing cells did not react to the insult. However, a small number of nestin-expressing cells in all inner ear tissues exhibited mitotic activity, supporting progenitor cell potential, though perhaps not involved in hair cell regeneration.
(Published by Elsevier B.V.)

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P30 DK116073 United States DK NIDDK NIH HHS; P30 NS048154 United States NS NINDS NIH HHS; R01 DC013912 United States DC NIDCD NIH HHS; T32 DC012280 United States DC NIDCD NIH HHS

Keywords: Crista; Hair cell regeneration; Nestin; Organ of corti; Saccule; Utricle

0 (Nestin)

Date Created: 20240131 Date Completed: 20240223 Latest Revision: 20240923

20240924

PMC10922748

10.1016/j.heares.2024.108962

38295585