Hi-Tag: a simple and efficient method for identifying protein-mediated long-range chromatin interactions with low cell numbers.

Publisher: Science China Press, co-published with Springer Country of Publication: China NLM ID: 101529880 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1869-1889 (Electronic) Linking ISSN: 16747305 NLM ISO Abbreviation: Sci China Life Sci Subsets: MEDLINE

Original Publication: Beijing : Science China Press, co-published with Springer

Chromatin*/metabolism ; Chromatin*/genetics; Humans ; Binding Sites ; Protein Binding ; Transposases/metabolism ; Transposases/genetics ; Chromatin Immunoprecipitation/methods ; Animals

Protein-mediated chromatin interactions can be revealed by coupling proximity-based ligation with chromatin immunoprecipitation. However, these techniques require complex experimental procedures and millions of cells per experiment, which limits their widespread application in life science research. Here, we develop a novel method, Hi-Tag, that identifies high-resolution, long-range chromatin interactions through transposase tagmentation and chromatin proximity ligation (with a phosphorothioate-modified linker). Hi-Tag can be implemented using as few as 100,000 cells, involving simple experimental procedures that can be completed within 1.5 days. Meanwhile, Hi-Tag is capable of using its own data to identify the binding sites of specific proteins, based on which, it can acquire accurate interaction information. Our results suggest that Hi-Tag has great potential for advancing chromatin interaction studies, particularly in the context of limited cell availability.
(© 2024. Science China Press.)

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Keywords: Hi-Tag; chromatin interactions; chromatin proximity ligation; low cell numbers; transposase tagmentation

0 (Chromatin)
EC 2.7.7.- (Transposases)

Date Created: 20240127 Date Completed: 20240506 Latest Revision: 20240506

20240507

10.1007/s11427-023-2441-0

38280143