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Hi-Tag: a simple and efficient method for identifying protein-mediated long-range chromatin interactions with low cell numbers.
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- Author(s): Qi X;Qi X; Zhang L; Zhang L; Zhao Q; Zhao Q; Zhou P; Zhou P; Zhang S; Zhang S; Li J; Li J; Zheng Z; Zheng Z; Xiang Y; Xiang Y; Dai X; Dai X; Jin Z; Jin Z; Jian Y; Jian Y; Li X; Li X; Li X; Li X; Fu L; Fu L; Fu L; Zhao S; Zhao S; Zhao S; Zhao S
- Source:
Science China. Life sciences [Sci China Life Sci] 2024 May; Vol. 67 (5), pp. 1027-1034. Date of Electronic Publication: 2024 Jan 23.- Publication Type:
Journal Article- Language:
English - Source:
- Additional Information
- Source: Publisher: Science China Press, co-published with Springer Country of Publication: China NLM ID: 101529880 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1869-1889 (Electronic) Linking ISSN: 16747305 NLM ISO Abbreviation: Sci China Life Sci Subsets: MEDLINE
- Publication Information: Original Publication: Beijing : Science China Press, co-published with Springer
- Subject Terms:
- Abstract: Protein-mediated chromatin interactions can be revealed by coupling proximity-based ligation with chromatin immunoprecipitation. However, these techniques require complex experimental procedures and millions of cells per experiment, which limits their widespread application in life science research. Here, we develop a novel method, Hi-Tag, that identifies high-resolution, long-range chromatin interactions through transposase tagmentation and chromatin proximity ligation (with a phosphorothioate-modified linker). Hi-Tag can be implemented using as few as 100,000 cells, involving simple experimental procedures that can be completed within 1.5 days. Meanwhile, Hi-Tag is capable of using its own data to identify the binding sites of specific proteins, based on which, it can acquire accurate interaction information. Our results suggest that Hi-Tag has great potential for advancing chromatin interaction studies, particularly in the context of limited cell availability.
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- Accession Number: 0 (Chromatin)
EC 2.7.7.- (Transposases) - Publication Date: Date Created: 20240127 Date Completed: 20240506 Latest Revision: 20240506
- Publication Date: 20240507
- Accession Number: 10.1007/s11427-023-2441-0
- Accession Number: 38280143
- Source:
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