The gene expression landscape of the human locus coeruleus revealed by single-nucleus and spatially-resolved transcriptomics.

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  • Additional Information
    • Source:
      Publisher: eLife Sciences Publications, Ltd Country of Publication: England NLM ID: 101579614 Publication Model: Electronic Cited Medium: Internet ISSN: 2050-084X (Electronic) Linking ISSN: 2050084X NLM ISO Abbreviation: Elife Subsets: MEDLINE
    • Publication Information:
      Original Publication: Cambridge, UK : eLife Sciences Publications, Ltd., 2012-
    • Subject Terms:
      Locus Coeruleus* ; Solitary Nucleus*; Humans ; Gene Expression Profiling ; Central Nervous System ; Norepinephrine ; Gene Expression
    • Abstract:
      Norepinephrine (NE) neurons in the locus coeruleus (LC) make long-range projections throughout the central nervous system, playing critical roles in arousal and mood, as well as various components of cognition including attention, learning, and memory. The LC-NE system is also implicated in multiple neurological and neuropsychiatric disorders. Importantly, LC-NE neurons are highly sensitive to degeneration in both Alzheimer's and Parkinson's disease. Despite the clinical importance of the brain region and the prominent role of LC-NE neurons in a variety of brain and behavioral functions, a detailed molecular characterization of the LC is lacking. Here, we used a combination of spatially-resolved transcriptomics and single-nucleus RNA-sequencing to characterize the molecular landscape of the LC region and the transcriptomic profile of LC-NE neurons in the human brain. We provide a freely accessible resource of these data in web-accessible and downloadable formats.
      Competing Interests: LW, HD, SK, AS, KM, MT, RB, JK, SP, TH, LC, KM, KM, SH No competing interests declared, MT The authors declare that they have no competing interests. Matthew N. Tran (MNT) is now a full-time employee at 23andMe and whose current work is unrelated to the contents of this manuscript. His contributions to this manuscript were made while previously employed at the Lieber Institute for Brain Development (LIBD)
      (© 2023, Weber, Divecha et al.)
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    • Grant Information:
      U01MH122849 United States NH NIH HHS; U01 MH122849 United States MH NIMH NIH HHS; R00 HG012229 United States HG NHGRI NIH HHS; K99 HG012229 United States HG NHGRI NIH HHS; K99HG012229 United States NH NIH HHS; R01DA053581 United States NH NIH HHS; R01 DA053581 United States DA NIDA NIH HHS
    • Contributed Indexing:
      Keywords: genetics; genomics; human; locus coeruleus; neuroscience; norepinephrine; postmortem human tissue; single-nucleus RNA-sequencing; spatially-resolved transcriptomics
    • Accession Number:
      X4W3ENH1CV (Norepinephrine)
    • Publication Date:
      Date Created: 20240124 Date Completed: 20240126 Latest Revision: 20241115
    • Publication Date:
      20241115
    • Accession Number:
      PMC10945708
    • Accession Number:
      10.7554/eLife.84628
    • Accession Number:
      38266073