Toward the production of block copolymers in microbial cells: achievements and perspectives.

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  • Author(s): Matsumoto K;Matsumoto K
  • Source:
    Applied microbiology and biotechnology [Appl Microbiol Biotechnol] 2024 Jan 22; Vol. 108 (1), pp. 164. Date of Electronic Publication: 2024 Jan 22.
  • Publication Type:
    Journal Article; Review
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Springer International Country of Publication: Germany NLM ID: 8406612 Publication Model: Electronic Cited Medium: Internet ISSN: 1432-0614 (Electronic) Linking ISSN: 01757598 NLM ISO Abbreviation: Appl Microbiol Biotechnol Subsets: MEDLINE
    • Publication Information:
      Original Publication: Berlin ; New York : Springer International, c1984-
    • Subject Terms:
    • Abstract:
      The microbial production of polyhydroxyalkanoate (PHA) block copolymers has attracted research interests because they can be expected to exhibit excellent physical properties. Although post-polymerization conjugation and/or extension have been used for PHA block copolymer synthesis, the discovery of the first sequence-regulating PHA synthase, PhaC AR , enabled the direct synthesis of PHA-PHA type block copolymers in microbial cells. PhaC AR spontaneously synthesizes block copolymers from a mixture of substrates. To date, Escherichia coli and Ralstonia eutropha have been used as host strains, and therefore, sequence regulation is not a host-specific phenomenon. The monomer sequence greatly influences the physical properties of the polymer. For example, a random copolymer of 3-hydroxybutyrate and 2-hydroxybutyrate deforms plastically, while a block copolymer of approximately the same composition exhibits elastic deformation. The structure of the PHA block copolymer can be expanded by in vitro evolution of the sequence-regulating PHA synthase. An engineered variant of PhaC AR can synthesize poly(D-lactate) as a block copolymer component, which allows for greater flexibility in the molecular design of block copolymers. Therefore, creating sequence-regulating PHA synthases with a further broadened substrate range will expand the variety of properties of PHA materials. This review summarizes and discusses the sequence-regulating PHA synthase, analytical methods for verifying block sequence, properties of block copolymers, and mechanisms of sequence regulation. KEY POINTS: • Spontaneous monomer sequence regulation generates block copolymers • Poly(D-lactate) segment can be synthesized using a block copolymerization system • Block copolymers exhibit characteristic properties.
      (© 2024. The Author(s).)
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    • Grant Information:
      JPMJMI19EB JST-Mirai Program; 20H04368 Japan Society for the Promotion of Science London
    • Contributed Indexing:
      Keywords: Biodegradable plastic; Block copolyester; PDLA; PHA synthase; Polyhydroxybutyrate
    • Accession Number:
      33X04XA5AT (Lactic Acid)
      TZP1275679 (3-Hydroxybutyric Acid)
      0 (Polyhydroxyalkanoates)
    • Publication Date:
      Date Created: 20240122 Date Completed: 20240123 Latest Revision: 20241023
    • Publication Date:
      20241023
    • Accession Number:
      PMC10803391
    • Accession Number:
      10.1007/s00253-023-12973-8
    • Accession Number:
      38252290