Item request has been placed!
×
Item request cannot be made.
×
Processing Request
Toward the production of block copolymers in microbial cells: achievements and perspectives.
Item request has been placed!
×
Item request cannot be made.
×
Processing Request
- Additional Information
- Source:
Publisher: Springer International Country of Publication: Germany NLM ID: 8406612 Publication Model: Electronic Cited Medium: Internet ISSN: 1432-0614 (Electronic) Linking ISSN: 01757598 NLM ISO Abbreviation: Appl Microbiol Biotechnol Subsets: MEDLINE
- Publication Information:
Original Publication: Berlin ; New York : Springer International, c1984-
- Subject Terms:
- Abstract:
The microbial production of polyhydroxyalkanoate (PHA) block copolymers has attracted research interests because they can be expected to exhibit excellent physical properties. Although post-polymerization conjugation and/or extension have been used for PHA block copolymer synthesis, the discovery of the first sequence-regulating PHA synthase, PhaC AR , enabled the direct synthesis of PHA-PHA type block copolymers in microbial cells. PhaC AR spontaneously synthesizes block copolymers from a mixture of substrates. To date, Escherichia coli and Ralstonia eutropha have been used as host strains, and therefore, sequence regulation is not a host-specific phenomenon. The monomer sequence greatly influences the physical properties of the polymer. For example, a random copolymer of 3-hydroxybutyrate and 2-hydroxybutyrate deforms plastically, while a block copolymer of approximately the same composition exhibits elastic deformation. The structure of the PHA block copolymer can be expanded by in vitro evolution of the sequence-regulating PHA synthase. An engineered variant of PhaC AR can synthesize poly(D-lactate) as a block copolymer component, which allows for greater flexibility in the molecular design of block copolymers. Therefore, creating sequence-regulating PHA synthases with a further broadened substrate range will expand the variety of properties of PHA materials. This review summarizes and discusses the sequence-regulating PHA synthase, analytical methods for verifying block sequence, properties of block copolymers, and mechanisms of sequence regulation. KEY POINTS: • Spontaneous monomer sequence regulation generates block copolymers • Poly(D-lactate) segment can be synthesized using a block copolymerization system • Block copolymers exhibit characteristic properties.
(© 2024. The Author(s).)
- References:
Sci Rep. 2021 Nov 17;11(1):22446. (PMID: 34789822)
Front Bioeng Biotechnol. 2022 Jul 19;10:946085. (PMID: 35928944)
Appl Microbiol Biotechnol. 2010 Jul;87(4):1427-35. (PMID: 20422180)
Biomacromolecules. 2019 Sep 9;20(9):3253-3260. (PMID: 31062966)
Front Bioeng Biotechnol. 2020 Dec 08;8:612991. (PMID: 33364233)
Biosci Biotechnol Biochem. 2022 Jan 24;86(2):217-223. (PMID: 34788370)
J Biotechnol. 2011 Jan 10;151(1):94-101. (PMID: 21111011)
Appl Microbiol Biotechnol. 2015 Nov;99(22):9555-63. (PMID: 26109003)
Biomacromolecules. 2006 Jun;7(6):1904-11. (PMID: 16768413)
Appl Microbiol Biotechnol. 2012 Mar;93(5):1917-25. (PMID: 22127753)
Front Bioeng Biotechnol. 2022 Jun 23;10:919969. (PMID: 35814019)
ACS Macro Lett. 2013 Nov 19;2(11):996-1000. (PMID: 35581867)
Biomacromolecules. 2018 Jul 9;19(7):2889-2895. (PMID: 29667817)
Microb Cell Fact. 2022 May 14;21(1):84. (PMID: 35568875)
Front Bioeng Biotechnol. 2019 Jul 24;7:177. (PMID: 31428606)
Macromol Chem Phys. 2020 May 19;221(12):. (PMID: 34404982)
Appl Microbiol Biotechnol. 2023 Dec;107(24):7557-7569. (PMID: 37773219)
Proc Natl Acad Sci U S A. 2008 Nov 11;105(45):17323-7. (PMID: 18978031)
Biomacromolecules. 2009 Dec 14;10(12):3176-81. (PMID: 19919068)
Front Bioeng Biotechnol. 2022 Dec 05;10:1057067. (PMID: 36545679)
Biomacromolecules. 2013 Mar 11;14(3):862-70. (PMID: 23351169)
Int J Biol Macromol. 2021 Sep 1;186:414-423. (PMID: 34246679)
Int J Biomater. 2012;2012:390947. (PMID: 22844286)
Front Bioeng Biotechnol. 2020 Oct 22;8:954. (PMID: 33195110)
Metab Eng Commun. 2022 Apr 30;14:e00199. (PMID: 35571351)
Int J Biol Macromol. 2020 Sep 15;159:250-257. (PMID: 32417540)
Front Bioeng Biotechnol. 2020 Aug 07;8:944. (PMID: 32903820)
Bioresour Technol. 2021 Dec;341:125873. (PMID: 34523584)
Biomacromolecules. 2018 Feb 12;19(2):662-671. (PMID: 29323923)
Appl Microbiol Biotechnol. 2012 Apr;94(2):365-76. (PMID: 22249718)
J Biosci Bioeng. 2016 Oct;122(4):406-14. (PMID: 27067372)
Int J Biol Macromol. 2021 Jan 15;167:1290-1296. (PMID: 33202278)
Biomacromolecules. 2009 Apr 13;10(4):682-5. (PMID: 19226108)
Int J Biol Macromol. 2023 Apr 15;234:123715. (PMID: 36801278)
Appl Microbiol Biotechnol. 2011 Apr;90(2):659-69. (PMID: 21181145)
Biomacromolecules. 2009 Apr 13;10(4):677-81. (PMID: 19249863)
Front Bioeng Biotechnol. 2020 Apr 03;8:257. (PMID: 32318554)
Appl Microbiol Biotechnol. 1997 Feb;47(2):140-3. (PMID: 9077002)
Microb Cell Fact. 2015 Nov 23;14:187. (PMID: 26597300)
Biophys Chem. 2023 May;296:107001. (PMID: 36913888)
Int J Biol Macromol. 2022 Nov 30;221:1184-1201. (PMID: 36113591)
Materials (Basel). 2021 Sep 13;14(18):. (PMID: 34576477)
Materials (Basel). 2022 Jun 17;15(12):. (PMID: 35744371)
Polymers (Basel). 2023 Jul 31;15(15):. (PMID: 37571152)
Metab Eng. 2013 Jan;15:159-66. (PMID: 23202750)
Appl Environ Microbiol. 2002 May;68(5):2411-9. (PMID: 11976116)
Biomacromolecules. 2022 Mar 14;23(3):1221-1231. (PMID: 34991313)
ACS Macro Lett. 2023 May 16;12(5):619-625. (PMID: 37094112)
J Biotechnol. 2007 Nov 1;132(3):296-302. (PMID: 17566581)
Microb Cell Fact. 2012 Apr 05;11:44. (PMID: 22480145)
Appl Microbiol Biotechnol. 2013 Jun;97(11):4821-9. (PMID: 23474615)
Appl Microbiol Biotechnol. 2013 Apr;97(8):3441-7. (PMID: 22801709)
Acc Chem Res. 2014 Aug 19;47(8):2390-6. (PMID: 24852135)
Biomacromolecules. 2011 Jul 11;12(7):2660-6. (PMID: 21618968)
- Grant Information:
JPMJMI19EB JST-Mirai Program; 20H04368 Japan Society for the Promotion of Science London
- Contributed Indexing:
Keywords: Biodegradable plastic; Block copolyester; PDLA; PHA synthase; Polyhydroxybutyrate
- Accession Number:
33X04XA5AT (Lactic Acid)
TZP1275679 (3-Hydroxybutyric Acid)
0 (Polyhydroxyalkanoates)
- Publication Date:
Date Created: 20240122 Date Completed: 20240123 Latest Revision: 20241023
- Publication Date:
20241023
- Accession Number:
PMC10803391
- Accession Number:
10.1007/s00253-023-12973-8
- Accession Number:
38252290
No Comments.