An RNA Motif That Enables Optozyme Control and Light-Dependent Gene Expression in Bacteria and Mammalian Cells.

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  • Additional Information
    • Source:
      Publisher: WILEY-VCH Country of Publication: Germany NLM ID: 101664569 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2198-3844 (Electronic) Linking ISSN: 21983844 NLM ISO Abbreviation: Adv Sci (Weinh) Subsets: MEDLINE
    • Publication Information:
      Original Publication: Weinheim : WILEY-VCH, [2014]-
    • Subject Terms:
      RNA*/genetics ; RNA, Catalytic*/chemistry ; RNA, Catalytic*/genetics ; RNA, Catalytic*/metabolism; Animals ; Nucleotide Motifs ; Bacteria/metabolism ; Gene Expression ; Mammals/metabolism
    • Abstract:
      The regulation of gene expression by light enables the versatile, spatiotemporal manipulation of biological function in bacterial and mammalian cells. Optoribogenetics extends this principle by molecular RNA devices acting on the RNA level whose functions are controlled by the photoinduced interaction of a light-oxygen-voltage photoreceptor with cognate RNA aptamers. Here light-responsive ribozymes, denoted optozymes, which undergo light-dependent self-cleavage and thereby control gene expression are described. This approach transcends existing aptamer-ribozyme chimera strategies that predominantly rely on aptamers binding to small molecules. The optozyme method thus stands to enable the graded, non-invasive, and spatiotemporally resolved control of gene expression. Optozymes are found efficient in bacteria and mammalian cells and usher in hitherto inaccessible optoribogenetic modalities with broad applicability in synthetic and systems biology.
      (© 2024 The Authors. Advanced Science published by Wiley‐VCH GmbH.)
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    • Grant Information:
      MA3442/5-1 German Research Council; MA3442/5-2 German Research Council; MA3442/9-1 German Research Council; MO2192/6-1 German Research Council; MO2192/6-2 German Research Council; MO2192/10-1 German Research Council; 615381 HORIZON EUROPE European Research Council
    • Contributed Indexing:
      Keywords: LOV‐domains; SELEX; aptamers; optogenetics; ribozymes; synthetic biology
    • Accession Number:
      63231-63-0 (RNA)
      0 (RNA, Catalytic)
    • Publication Date:
      Date Created: 20240116 Date Completed: 20240328 Latest Revision: 20240329
    • Publication Date:
      20240329
    • Accession Number:
      PMC10966536
    • Accession Number:
      10.1002/advs.202304519
    • Accession Number:
      38227373