Prognostic impact of Ki-67 in canine splenic hemangiosarcoma: A preliminary study.

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  • Additional Information
    • Source:
      Publisher: Sage Country of Publication: United States NLM ID: 0312020 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1544-2217 (Electronic) Linking ISSN: 03009858 NLM ISO Abbreviation: Vet Pathol Subsets: MEDLINE
    • Publication Information:
      Publication: Jan. 2010- : Thousand Oaks, CA : Sage
      Original Publication: 1971- : Basel : New York : Karger.
    • Subject Terms:
      Hemangiosarcoma*/veterinary ; Hemangiosarcoma*/pathology ; Hemangiosarcoma*/metabolism ; Hemangiosarcoma*/diagnosis ; Ki-67 Antigen*/metabolism ; Dog Diseases*/pathology ; Dog Diseases*/metabolism ; Dog Diseases*/diagnosis ; Splenic Neoplasms*/veterinary ; Splenic Neoplasms*/pathology ; Splenic Neoplasms*/diagnosis ; Splenic Neoplasms*/metabolism; Animals ; Dogs ; Prognosis ; Male ; Female ; Immunohistochemistry/veterinary ; Splenectomy/veterinary ; Mitotic Index/veterinary ; Neoplasm Staging/veterinary ; Biomarkers, Tumor/metabolism
    • Abstract:
      Canine splenic hemangiosarcoma has a high metastatic rate and short survival time. Currently, the main prognostic parameters are tumor stage and therapy, while data on histologic parameters, such as grade and Ki-67 expression, are scarce. The aims of this study were to compare two methods of assessment of Ki-67, verify their prognostic impact, and define a threshold value based on survival. Thirty-one cases of histologically diagnosed canine splenic hemangiosarcoma, which were treated with splenectomy and had full staging and follow-up information, were collected. Three were stage I, 17 stage II, and 11 stage III. The mean mitotic count (MC) was 23.9 (standard deviation [SD]: 22.1) and the median was 15 (range, 1-93). Immunohistochemistry for Ki-67 was performed, the Ki-67 labeling index (Ki-67LI) was assessed as a percentage of positive neoplastic nuclei per ≥500 cell, and the Ki-67 count (KI-67C) was defined as the average number of positive nuclei using a 1 cm 2 optical grid performed in 5, 40× fields. The mean Ki-67LI and Ki-67C were 56.4% (SD: 38.7) and 27.2 (SD: 12.9) and medians were 51% (range, 8.2-55.2) and 26 (range, 5.5-148), respectively. Using a cut-off of 56% and 9, respectively, Kaplan-Meier survival curves showed an association of overall survival with Ki-67LI and MC. In addition to clinical stage, Ki-67LI maintained its prognostic value on multivariate analysis, supporting the role of Ki-67LI as an independent prognostic parameter. Based on these results, we propose a diagnostically applicable cut-off value of 56% for Ki-67LI as a prognostic parameter for canine splenic hemangiosarcoma.
      Competing Interests: Declaration of Conflicting InterestsThe author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.
    • Contributed Indexing:
      Keywords: Ki-67; dogs; hemangiosarcoma; histopathology; immunohistochemistry; prognosis; spleen
    • Accession Number:
      0 (Ki-67 Antigen)
      0 (Biomarkers, Tumor)
    • Publication Date:
      Date Created: 20240112 Date Completed: 20240701 Latest Revision: 20240724
    • Publication Date:
      20240726
    • Accession Number:
      10.1177/03009858231225507
    • Accession Number:
      38214328