Metalloproteomic Investigation of Hg-Binding Proteins in Renal Tissue of Rats Exposed to Mercury Chloride.

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  • Additional Information
    • Source:
      Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE
    • Publication Information:
      Original Publication: Basel, Switzerland : MDPI, [2000-
    • Subject Terms:
      Acid-Base Imbalance* ; Mercury*/toxicity; Animals ; Rats ; Carrier Proteins ; Chlorides ; Proteomics ; Mercuric Chloride/toxicity ; Biomarkers
    • Abstract:
      Results obtained from rat studies indicate that, even at low concentrations, mercurial species cause harmful effects on the kidneys, by inducing the nephrotic oxidative stress response. In the present work, Hg-associated proteins were identified as possible mercury-exposure biomarkers in rat kidneys exposed to low mercury chloride concentrations for 30 days (Hg-30) and 60 days (Hg-60), using metalloproteomic strategies. The renal proteomic profile was fractioned by two-dimensional electrophoresis and the mercury determinations in kidney samples, protein pellets and protein spots were performed using graphite furnace atomic absorption spectrometry. The characterization of Hg-associated protein spots and the analysis of differentially expressed proteins were performed by liquid chromatography, coupled with tandem mass spectrometry. Eleven Hg-associated protein spots with a concentration range of 79 ± 1 to 750 ± 9 mg kg -1 in the Hg-60 group were identified. The characterization and expression analyses allowed the identification of 53 proteins that were expressed only in the Hg-60 group, 13 "upregulated" proteins ( p > 0.95) and 47 "downregulated" proteins ( p < 0.05). Actin isoforms and hemoglobin subunits were identified in protein spots of the Hg-60 group, with mercury concentrations in the range of 138 to 750 mg kg -1 , which qualifies these proteins as potential mercury-exposure biomarkers.
      Competing Interests: The authors declare no conflict of interest.
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    • Grant Information:
      306440/2022-9, 30478/2018-9 and 404485/2016-2 National Council for Scientific and Technological Development; 2017/09466-3 and 2019/02538-4 São Paulo Research Foundation; CAPES-Print AUXPE-Process: 88881.3107432018-0.1 Coordenação de Aperfeicoamento de Pessoal de Nível Superior; 0282/19 Financiadora de Estudos e Projetos
    • Contributed Indexing:
      Keywords: LC-MS/MS; mercurial species; mercury bioaccumulation; mercury biomarkers; metallomics
    • Accession Number:
      0 (Carrier Proteins)
      0 (Chlorides)
      53GH7MZT1R (Mercuric Chloride)
      FXS1BY2PGL (Mercury)
      0 (Biomarkers)
    • Publication Date:
      Date Created: 20240111 Date Completed: 20240112 Latest Revision: 20240113
    • Publication Date:
      20240113
    • Accession Number:
      PMC10779082
    • Accession Number:
      10.3390/ijms25010164
    • Accession Number:
      38203335