Role of folic acid in regulating gut microbiota and short-chain fatty acids based on an in vitro fermentation model.

Publisher: Springer International Country of Publication: Germany NLM ID: 8406612 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1432-0614 (Electronic) Linking ISSN: 01757598 NLM ISO Abbreviation: Appl Microbiol Biotechnol Subsets: MEDLINE

Original Publication: Berlin ; New York : Springer International, c1984-

Folic Acid Deficiency* ; Gastrointestinal Microbiome*; Animals ; Humans ; Folic Acid ; Fermentation ; RNA, Ribosomal, 16S/genetics ; Reproducibility of Results ; Fatty Acids, Volatile

Folic acid deficiency is common worldwide and is linked to an imbalance in gut microbiota. However, based on model animals used to study the utilization of folic acid by gut microbes, there are challenges of reproducibility and individual differences. In this study, an in vitro fecal slurry culture model of folic acid deficiency was established to investigate the effects of supplementation with 5-methyltetrahydrofolate (MTHF) and non-reduced folic acid (FA) on the modulation of gut microbiota. 16S rRNA sequencing results revealed that both FA (29.7%) and MTHF (27.9%) supplementation significantly reduced the relative abundance of Bacteroidetes compared with control case (34.3%). MTHF supplementation significantly improved the relative abundance of Firmicutes by 4.49%. Notably, compared with the control case, FA and MTHF supplementation promoted an increase in fecal levels of Lactobacillus, Bifidobacterium, and Pediococcus. Short-chain fatty acid (SCFA) analysis showed that folic acid supplementation decreased acetate levels and increased fermentative production of isobutyric acid. The in vitro fecal slurry culture model developed in this study can be utilized as a model of folic acid deficiency in humans to study the gut microbiota and demonstrate that exogenous folic acid affects the composition of the gut microbiota and the level of SCFAs. KEY POINTS: • Establishment of folic acid deficiency in an in vitro culture model. • Folic acid supplementation regulates intestinal microbes and SCFAs. • Connections between microbes and SCFAs after adding folic acid are built.
(© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)

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31972974 National Natural Science Foundation of China

Keywords: 5-Methyltetrahydrofolate; Folic acid deficiency; In vitro fecal slurry culture model; Microbial community; Non-reduced folic acid; SCFAs

935E97BOY8 (Folic Acid)
0 (RNA, Ribosomal, 16S)
0 (Fatty Acids, Volatile)

Date Created: 20240104 Date Completed: 20240105 Latest Revision: 20240117

20240117

10.1007/s00253-023-12825-5

38175236