RNF187 governs the maintenance of mouse GC-2 cell development by facilitating histone H3 ubiquitination at K57/80.

Publisher: Shanghai Materia Medica Country of Publication: China NLM ID: 100942132 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1745-7262 (Electronic) Linking ISSN: 1008682X NLM ISO Abbreviation: Asian J Androl Subsets: MEDLINE

Publication: Shanghai : Shanghai Materia Medica
Original Publication: Beijing : Science Press,

Histones*/metabolism ; Ubiquitination* ; Ubiquitin-Protein Ligases*/metabolism ; Ubiquitin-Protein Ligases*/genetics ; Cell Proliferation*/physiology ; Cell Proliferation*/genetics; Animals ; Mice ; Male ; Cell Line ; Spermatocytes/metabolism ; Cell Movement/genetics ; Cell Movement/physiology ; Lysine/metabolism ; Cell Survival/physiology ; Cell Survival/genetics

RING finger 187 (RNF187), a ubiquitin-ligating (E3) enzyme, plays a crucial role in the proliferation of cancer cells. However, it remains unclear whether RNF187 exhibits comparable functionality in the development of germline cells. To investigate the potential involvement of RNF187 in germ cell development, we conducted interference and overexpression assays using GC-2 cells, a mouse spermatocyte-derived cell line. Our findings reveal that the interaction between RNF187 and histone H3 increases the viability, proliferation, and migratory capacity of GC-2 cells. Moreover, we provide evidence demonstrating that RNF187 interacts with H3 and mediates the ubiquitination of H3 at lysine 57 (K57) or lysine 80 (K80), directly or indirectly resulting in increased cellular transcription. This is a study to report the role of RNF187 in maintaining the development of GC-2 cells by mediating histone H3 ubiquitination, thus highlighting the involvement of the K57 and K80 residues of H3 in the epistatic regulation of gene transcription. These discoveries provide a new theoretical foundation for further comprehensive investigations into the function of RNF187 in the reproductive system.
(Copyright © 2023 Copyright: © The Author(s)(2023).)

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0 (Histones)
EC 2.3.2.27 (Ubiquitin-Protein Ligases)
K3Z4F929H6 (Lysine)

Date Created: 20231229 Date Completed: 20240729 Latest Revision: 20240729

20240730

PMC11156453

10.4103/aja202368

38156805