Integration of miRNA in exosomes and single-cell RNA-seq profiles in endemic osteoarthritis, Kashin-Beck disease.

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  • Additional Information
    • Source:
      Publisher: Ios Press Country of Publication: Netherlands NLM ID: 8807441 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1872-8081 (Electronic) Linking ISSN: 09516433 NLM ISO Abbreviation: Biofactors Subsets: MEDLINE
    • Publication Information:
      Publication: Amsterdam : Ios Press
      Original Publication: Oxford ; Washington, DC : Published for the International Union of Biochemistry by IRL Press, [c1988-
    • Subject Terms:
      Chondrocytes*/metabolism ; Exosomes*/genetics ; Exosomes*/metabolism ; Kashin-Beck Disease*/blood ; Kashin-Beck Disease*/genetics ; Kashin-Beck Disease*/metabolism ; Kashin-Beck Disease*/pathology ; MicroRNAs*/genetics ; MicroRNAs*/blood ; Single-Cell Gene Expression Analysis*/methods; Aged ; Female ; Humans ; Male ; Middle Aged ; Gene Expression Regulation ; RNA-Seq
    • Abstract:
      Kashin-Beck disease (KBD) is an endemic, chronic degenerative joint disease in China. Exosomes miRNAs, as signaling molecules in intercellular communication, can transfer specific biological martials into target cell to regulate their function and might participate in the pathogenesis of KBD. We isolated serum and chondrocytes-derived exosomes, miRNA sequencing revealed exosomes miRNA profiles and differentially expressed miRNAs (DE-miRNAs) were identified. The target genes were predicted of known and novel DE-miRNAs with TargetScan 5.0 and miRanda 3.3a database. Single-cell RNA sequencing (scRNA-seq) was performed to identify chondrocyte clusters and their gene signatures in KBD. And we performed comparative analysis between the serum and chondrocytes-derived exosomes DE-miRNA target genes and differentially expressed genes of each cell clusters. A total of 20 DE-miRNAs were identified in serum-derived exosomes. In the miRNA expression of chondrocytes-derived exosomes, 53 DE-miRNAs were identified. 16,063 predicted targets were identified as the target genes in the serum-derived exosomes, 57,316 predicted targets were identified as the target genes in the chondrocytes-derived exosomes. Seven clusters were labeled by cell type according to the expression of previously described markers. Three hundred fifteen common genes were found among serum/chondrocytes-derived exosomes DE-miRNA target genes and DEGs identified by scRNA-seq analysis. We firstly integratly analyzed the serum and chondrocytes exosomes miRNA with single-cell RNA sequencing (scRNA-seq) data of KBD chondrocyte, the results showed that DE-miRNAs in exosomes might play a potential role in regulating genes expression in different KBD chondrocytes clusters by exosomes mediating cell-cell communications functions, which could improve the new diagnosis and treatment methods for KBD.
      (© 2023 International Union of Biochemistry and Molecular Biology.)
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    • Grant Information:
      2018BSHEDZZ96 Shaanxi Postdoctoral Foundation; 2018BSHYDZZ47 Shaanxi Postdoctoral Foundation; 2023-JC-YB-704 Natural Science Basic Research Plan in Shaanxi Province of China; 81620108026 National Natural Science Foundation of China; 82273752 National Natural Science Foundation of China; 82373700 National Natural Science Foundation of China; 2021M692543 China Postdoctoral Foundation; 2022M712526 China Postdoctoral Foundation; 23618504 NHC Key Laboratory of Etiology and Epidemiology
    • Contributed Indexing:
      Keywords: Kashin–Beck disease; exosomes; miRNA sequencing; single‐cell RNA sequencing
    • Accession Number:
      0 (MicroRNAs)
    • Publication Date:
      Date Created: 20231229 Date Completed: 20240808 Latest Revision: 20240809
    • Publication Date:
      20240812
    • Accession Number:
      10.1002/biof.2033
    • Accession Number:
      38156801