Generation of canine induced pluripotent stem cells under feeder-free conditions using Sendai virus vector encoding six canine reprogramming factors.

Publisher: Cell Press Country of Publication: United States NLM ID: 101611300 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2213-6711 (Electronic) Linking ISSN: 22136711 NLM ISO Abbreviation: Stem Cell Reports Subsets: MEDLINE

Original Publication: [Cambridge, MA] : Cell Press, c 2013-

Induced Pluripotent Stem Cells*; Animals ; Dogs ; Humans ; Cellular Reprogramming/genetics ; Sendai virus/genetics ; Kruppel-Like Factor 4 ; Feeder Cells ; Fibroblasts ; Cell Differentiation/genetics

Although it is in its early stages, canine induced pluripotent stem cells (ciPSCs) hold great potential for innovative translational research in regenerative medicine, developmental biology, drug screening, and disease modeling. However, almost all ciPSCs were generated from fibroblasts, and available canine cell sources for reprogramming are still limited. Furthermore, no report is available to generate ciPSCs under feeder-free conditions because of their low reprogramming efficiency. Here, we reanalyzed canine pluripotency-associated genes and designed canine LIN28A, NANOG, OCT3/4, SOX2, KLF4, and C-MYC encoding Sendai virus vector, called 159cf. and 162cf. We demonstrated that not only canine fibroblasts but also canine urine-derived cells, which can be isolated using a noninvasive and straightforward method, were successfully reprogrammed with or without feeder cells. ciPSCs existed in undifferentiated states, differentiating into the three germ layers in vitro and in vivo. We successfully generated ciPSCs under feeder-free conditions, which can promote studies in veterinary and consequently human regenerative medicines.
Competing Interests: Declaration of interests This study was also funded by Anicom Specialty Medical Institute. T.A. and K.W. are employees of Anicom Specialty Medical Institute. M.I. and M.N. are the inventors of the patent application (JP 6770224 B2, JP 7174958 B2, US 10544431 B1, EP 3246406 B1, TW I 696700 B, CN 107109400 B, SG 11201705820U, KR 6102459458 B1).
(Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.)

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Keywords: Sendai virus vectors; canine reprogramming genes; feeder-free; induced pluripotent stem cells; six reprogramming factors; urine-derived cells

0 (Kruppel-Like Factor 4)

Date Created: 20231222 Date Completed: 20240112 Latest Revision: 20241023

20241023

PMC10828825

10.1016/j.stemcr.2023.11.010

38134923