Functional roles and localization of hydrolases in the Japanese mitten crab Eriocheir japonica.

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  • Additional Information
    • Source:
      Publisher: Pergamon Country of Publication: England NLM ID: 9516061 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-1107 (Electronic) Linking ISSN: 10964959 NLM ISO Abbreviation: Comp Biochem Physiol B Biochem Mol Biol Subsets: MEDLINE
    • Publication Information:
      Publication: Oxford : Pergamon
      Original Publication: Oxford : Tarrytown, NY : Pergamon ; Elsevier, c1994-
    • Subject Terms:
      Hydrolases* ; Brachyura*; Humans ; Animals ; Japan ; Seafood ; Rivers ; Esters
    • Abstract:
      The Japanese mitten crab Eriocheir japonica inhabits rivers throughout Japan and is being cultivated for food. To conduct aquaculture efficiently, it is crucial to comprehend the physiological functions of the target organisms. However, there is a lack of fundamental information on Japanese mitten crabs. In this study, hydrolases were extracted from the midgut glands of Japanese mitten crabs and their metabolic activities were analyzed. An enzyme with hydrolytic activity was discovered within the cytosol of the midgut gland. Western blot analysis also revealed that the Japanese mitten crab contains a hydrolase with cross-reactivity to human carboxylesterase 1 (hCES1) antibodies. The substrate specificity of the S9 fraction of the midgut gland was investigated and, interestingly, it was revealed that it reacts well with indomethacin phenyl ester and fluorescein diacetate, which are substrates of hCES2, not substrates of hCES1. Furthermore, this enzyme was observed to metabolize the ester derivative of astaxanthin, which is a red pigment inherent to the Japanese mitten crab. These findings underscore the significance the midgut gland in the Japanese mitten crab as an important organ for metabolizing both endogenous and exogenous ester-type compounds.
      Competing Interests: Declaration of Competing Interest None.
      (Copyright © 2023 Elsevier Inc. All rights reserved.)
    • Contributed Indexing:
      Keywords: Astaxanthin; Carboxylesterase; Eriocheir japonica; Japanese mitten crab; Midgut gland; Substrate specificity
    • Accession Number:
      EC 3.- (Hydrolases)
      0 (Esters)
    • Publication Date:
      Date Created: 20231214 Date Completed: 20240110 Latest Revision: 20240110
    • Publication Date:
      20240110
    • Accession Number:
      10.1016/j.cbpb.2023.110932
    • Accession Number:
      38097062