Genome-wide in-locus epitope tagging of Arabidopsis proteins using prime editors.

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
Read More Add to Saved list
  • Additional Information
    • Source:
      Publisher: Korean Society for Biochemistry and Molecular Biology Country of Publication: Korea (South) NLM ID: 101465334 Publication Model: Print Cited Medium: Internet ISSN: 1976-670X (Electronic) Linking ISSN: 19766696 NLM ISO Abbreviation: BMB Rep Subsets: MEDLINE
    • Publication Information:
      Original Publication: Seoul : Korean Society for Biochemistry and Molecular Biology
    • Subject Terms:
      CRISPR-Cas Systems*/genetics ; Arabidopsis Proteins*/genetics; Gene Editing ; RNA, Guide, CRISPR-Cas Systems ; DNA/genetics
    • Abstract:
      Prime editors (PEs), which are CRISPR-Cas9 nickase (H840A)-reverse transcriptase fusion proteins programmed with prime editing guide RNAs (pegRNAs), can not only edit bases but also install transversions, insertions, or deletions without both donor DNA and double-strand breaks at the target DNA. As the demand for in-locus tagging is increasing, to reflect gene expression dynamics influenced by endogenous genomic contexts, we demonstrated that PEs can be used to introduce the hemagglutinin (HA) epitope tag to a target gene locus, enabling molecular and biochemical studies using in-locus tagged plants. To promote genome-wide in-locus tagging, we also implemented a publicly available database that designs pegRNAs for in-locus tagging of all the Arabidopsis genes. [BMB Reports 2024; 57(1): 66-70].
    • References:
      Mol Plant. 2020 Dec 7;13(12):1663-1665. (PMID: 33189907)
      Plant Methods. 2021 Feb 23;17(1):21. (PMID: 33622383)
      Plant J. 1998 Dec;16(6):735-43. (PMID: 10069079)
      Plant Biotechnol J. 2020 Nov;18(11):2167-2169. (PMID: 32372479)
      Mol Plant. 2020 May 4;13(5):675-678. (PMID: 32234340)
      Genome Biol. 2020 Oct 06;21(1):257. (PMID: 33023639)
      Natl Sci Rev. 2019 May;6(3):394-396. (PMID: 34691888)
      Nat Biotechnol. 2020 May;38(5):582-585. (PMID: 32393904)
      Plant Sci. 2022 Mar;316:111162. (PMID: 35151447)
      Cell. 1998 Jun 26;93(7):1207-17. (PMID: 9657153)
      Mol Plant. 2020 May 4;13(5):671-674. (PMID: 32222486)
      Nat Biotechnol. 2023 Dec;41(12):1758-1764. (PMID: 36894598)
      Plant Commun. 2020 Apr 08;1(3):100043. (PMID: 33367239)
      Nucleic Acids Res. 2021 Jul 2;49(W1):W499-W504. (PMID: 33939828)
      Bioinformatics. 2015 Dec 15;31(24):4014-6. (PMID: 26358729)
      Mol Plant. 2020 May 4;13(5):667-670. (PMID: 32222487)
      Science. 2018 Sep 21;361(6408):1259-1262. (PMID: 30166441)
      Plant Biotechnol J. 2021 Mar;19(3):415-417. (PMID: 33091225)
      Nature. 2019 Dec;576(7785):149-157. (PMID: 31634902)
      Front Genome Ed. 2021 Mar 04;3:617553. (PMID: 34713247)
      Nat Commun. 2015 Dec 17;6:10237. (PMID: 26674669)
      Plant Biotechnol J. 2020 Dec;18(12):2370-2372. (PMID: 32415890)
      Nat Methods. 2018 Aug;15(8):598-600. (PMID: 29988096)
      Nat Plants. 2020 Jul;6(7):773-779. (PMID: 32601419)
    • Accession Number:
      0 (RNA, Guide, CRISPR-Cas Systems)
      0 (Arabidopsis Proteins)
      9007-49-2 (DNA)
    • Publication Date:
      Date Created: 20231206 Date Completed: 20240131 Latest Revision: 20240202
    • Publication Date:
      20240202
    • Accession Number:
      PMC10828436
    • Accession Number:
      38053291