Microglia modulate sleep/wakefulness under baseline conditions and under acute social defeat stress in adult mice.

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
Read More Add to Saved list
  • Additional Information
    • Source:
      Publisher: Elsevier Country of Publication: Ireland NLM ID: 8500749 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1872-8111 (Electronic) Linking ISSN: 01680102 NLM ISO Abbreviation: Neurosci Res Subsets: MEDLINE
    • Publication Information:
      Publication: Limerick : Elsevier
      Original Publication: [Shannon : Elsevier Scientific Publishers Ireland, c1984-
    • Subject Terms:
      Microglia*/metabolism ; Microglia*/physiology ; Wakefulness*/physiology ; Stress, Psychological*/physiopathology ; Stress, Psychological*/psychology ; Sleep*/physiology ; Mice, Inbred C57BL* ; Social Defeat* ; Electroencephalography*; Animals ; Male ; Mice ; Electromyography ; Receptors, Granulocyte-Macrophage Colony-Stimulating Factor/antagonists & inhibitors ; Brain ; Organic Chemicals
    • Abstract:
      Although sleep is tightly regulated by multiple neuronal circuits in the brain, nonneuronal cells such as glial cells have been increasingly recognized as crucial sleep regulators. Recent studies have shown that microglia may act to maintain wakefulness. Here, we investigated the possible involvement of microglia in the regulation of sleep quantity and quality under baseline and stress conditions through electroencephalography (EEG)/electromyography (EMG) recordings, and by employing pharmacological methods to eliminate microglial cells in the adult mouse brain. We found that severe microglial depletion induced by the colony-stimulating factor 1 receptor (CSF1R) antagonist PLX5622 (PLX) reversibly decreased the total wake time and the wake episode duration and increased the EEG slow-wave power during wakefulness under baseline conditions. To examine the role of microglia in sleep/wake regulation under mental stress, we used the acute social defeat stress (ASDS) paradigm, an ethological model for psychosocial stress. Sleep analysis under ASDS revealed that microglial depletion exacerbated the stress-induced decrease in the total wake time and increase in anxiety-like behaviors in the open field test. These results demonstrate that microglia actively modulate sleep quantity and architecture under both baseline and stress conditions. Our findings suggest that microglia may potentially provide resilience against acute psychosocial stress by regulating restorative sleep.
      Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
      (Copyright © 2023. Published by Elsevier B.V.)
    • Contributed Indexing:
      Keywords: Anxiety-like behavior; CSF1-CSF1R signalling; Microglia; Restorative sleep; Sleep/wakefulness; Social defeat stress
    • Accession Number:
      0 (PLX5622)
      0 (Csf1r protein, mouse)
      0 (Receptors, Granulocyte-Macrophage Colony-Stimulating Factor)
      0 (Organic Chemicals)
    • Publication Date:
      Date Created: 20231129 Date Completed: 20240510 Latest Revision: 20240510
    • Publication Date:
      20240511
    • Accession Number:
      10.1016/j.neures.2023.11.010
    • Accession Number:
      38029860