Extracellular and intracellular destruction of Pseudomonas aeruginosa by Dictyostelium discoideum phagocytes mobilize different antibacterial mechanisms.

Publisher: Blackwell Scientific Publications Country of Publication: England NLM ID: 8712028 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1365-2958 (Electronic) Linking ISSN: 0950382X NLM ISO Abbreviation: Mol Microbiol Subsets: MEDLINE

Original Publication: Oxford, OX ; Boston, MA : Blackwell Scientific Publications, c1987-

Dictyostelium*/metabolism ; Dictyostelium*/microbiology; Humans ; Pseudomonas aeruginosa/metabolism ; Phagosomes/metabolism ; Neutrophils ; Anti-Bacterial Agents/metabolism ; Bacteria

Ingestion and killing of bacteria by phagocytic cells are critical processes to protect the human body from bacterial infections. In addition, some immune cells (neutrophils, NK cells) can release microbicidal molecules in the extracellular medium to eliminate non-ingested microorganism. Molecular mechanisms involved in the resulting intracellular and extracellular killing are still poorly understood. In this study, we used the amoeba Dictyostelium discoideum as a model phagocyte to investigate the mechanisms allowing intracellular and extracellular killing of Pseudomonas aeruginosa. When a D. discoideum cell establishes a close contact with a P. aeruginosa bacterium, it can either ingest it and kill it in phagosomes, or kill it extracellularly, allowing a direct side-by-side comparison of these two killing modalities. Efficient intracellular destruction of P. aeruginosa requires the presence of the Kil2 pump in the phagosomal membrane. On the contrary, extracellular lysis is independent on Kil2 but requires the expression of the superoxide-producing protein NoxA, and the extracellular release of the AplA bacteriolytic protein. These results shed new light on the molecular mechanisms allowing elimination of P. aeruginosa bacteria by phagocytic cells.
(© 2023 The Authors. Molecular Microbiology published by John Wiley & Sons Ltd.)

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310030_201186 Switzerland SNSF_ Swiss National Science Foundation

Keywords: Dictyostelium discoideum; Pseudomonas aeruginosa; AplA; Kil2; NADPH oxidase; NoxA; amoebapore; bactericidal; killing

0 (Anti-Bacterial Agents)

Date Created: 20231129 Date Completed: 20240115 Latest Revision: 20240412

20250114

10.1111/mmi.15197

38017607