Production of polyhydroxybutyrate by coupled saccharification-fermentation of inulin.

Publisher: Springer-Verlag Country of Publication: Germany NLM ID: 101088505 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1615-7605 (Electronic) Linking ISSN: 16157591 NLM ISO Abbreviation: Bioprocess Biosyst Eng Subsets: MEDLINE

Original Publication: Berlin, Germany : Springer-Verlag, 2001-

Inulin*/metabolism ; Cupriavidus necator*; Fermentation ; Polyhydroxybutyrates ; Fructose ; Hydroxybutyrates

Inulin is a fructose-based polysaccharide that can be found in several plant species, from grass and onions to chicory roots; thus, it has the potential to be an excellent renewable source of fructose for several industrial applications. Among them, inulin hydrolysis can be coupled to a fermentation operation to produce polyhydroxybutyrate (PHB) using Cupriavidus necator H16. This work reports the PHB production process involving chicory root inulin hydrolysis using inulinase Novozym 960 followed by a C. necator fermentation. It was found that the maximum saccharification (95% wt.) was reached at 269 U/g inulin after 90 min. The hydrolysates obtained were then inoculated with C. necator, leading to a biomass concentration of 4 g/L with 30% (w/w) polymer accumulation. Although PHB production was low, during the first hours, the cell growth and polymer accumulation detected did not coincide with a fructose concentration decrease, suggesting a simultaneous saccharification and fermentation process, potentially alleviating the product inhibition inherent to the inulinase-fructose system. The characterization of the obtained PHB showed a polymer with more homogeneous values of M w , and better thermal stability than PHB produced using pure fructose as a fermentation substrate. The results obtained demonstrate a viable alternative carbon substrate for PHB production, opening the possibility for inulin-rich renewable feedstock valorization.
(© 2023. The Author(s).)

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PAPIIT IN219520 Dirección General de Asuntos del Personal Académico, Universidad Nacional Autónoma de México; IN214123 Dirección General de Asuntos del Personal Académico, Universidad Nacional Autónoma de México

Keywords: Bioplastics; Cupriavidus necator; Enzymatic hydrolysis; Inulin; Polyhydroxybutyrate

9005-80-5 (Inulin)
0 (Polyhydroxybutyrates)
30237-26-4 (Fructose)
0 (Hydroxybutyrates)

Date Created: 20231125 Date Completed: 20240110 Latest Revision: 20240112

20240112

PMC10776465

10.1007/s00449-023-02953-7

38006410