The Effects of Unilateral Labyrinthectomy on Monoamine Neurotransmitters in the Medial Vestibular Nucleus of Rats.

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  • Additional Information
    • Source:
      Publisher: MDPI Country of Publication: Switzerland NLM ID: 101596414 Publication Model: Electronic Cited Medium: Internet ISSN: 2218-273X (Electronic) Linking ISSN: 2218273X NLM ISO Abbreviation: Biomolecules Subsets: MEDLINE
    • Publication Information:
      Original Publication: Basel, Switzerland : MDPI, 2011-
    • Subject Terms:
      Histamine*/metabolism ; Vestibule, Labyrinth*; Rats ; Animals ; Rats, Sprague-Dawley ; Serotonin/metabolism ; Neurotransmitter Agents/metabolism ; Vestibular Nuclei/metabolism
    • Abstract:
      Background: This study aimed to investigate the effects of unilateral labyrinthectomy (UL) on monoamine neurotransmitters in the medial vestibular nucleus (MVN) of rats.
      Methods: Adult Sprague-Dawley rats were utilized for the vestibular impaired animal model through UL. The success of the model establishment and the recovery process were evaluated using vestibular behavioral tests, including spontaneous nystagmus, postural asymmetry, and balance beam test. Additionally, the expression levels of c-Fos protein in the MVN were assessed by immunofluorescence. Furthermore, changes in the expression levels of monoamine neurotransmitters, including 5-hydroxytryptamine (5-HT), norepinephrine (NE), dopamine (DA), and histamine in the MVN, were analyzed using high-performance liquid chromatography (HPLC) at different time points after UL (4 h, 8 h, 1 day, 2 days, 4 days, and 7 days).
      Results: Compared to the sham control group, the UL group exhibited the most pronounced vestibular impairment symptoms at 4 h post-UL, which significantly decreased at 4 days and almost fully recovered by 7 days. Immunofluorescence results showed a notable upregulation of c-Fos expression in the MVN subsequent to the UL-4 h, serving as a reliable indicator of heightened neuronal activity. In comparison with the sham group, HPLC analysis showed that the levels of 5-HT and NE in the ipsilesional MVN of the UL group were significantly elevated within 4 days after UL, and peaked on 1 day and 2 days, respectively. DA showed an increasing trend at different time points up to 7 days post-UL, while histamine levels significantly increased only at 1 day post-UL.
      Conclusions: UL-induced dynamic changes in monoamine neurotransmitters during the early compensation period in the rat MVN may be associated with the regulation of the central vestibular compensation mechanism by the MVN.
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    • Grant Information:
      82371168 National Natural Science Foundation of China; 82171152 National Natural Science Foundation of China; 2023BCB027 Hubei Provincial Key Research and Development Program; 2022020801010458 Wuhan Knowledge Innovation Project
    • Contributed Indexing:
      Keywords: high-performance liquid chromatography; medial vestibular nucleus; monoamine neurotransmitters; unilateral labyrinthectomy; vestibular compensation
    • Accession Number:
      820484N8I3 (Histamine)
      333DO1RDJY (Serotonin)
      0 (Neurotransmitter Agents)
    • Publication Date:
      Date Created: 20231125 Date Completed: 20231127 Latest Revision: 20240104
    • Publication Date:
      20240104
    • Accession Number:
      PMC10669524
    • Accession Number:
      10.3390/biom13111637
    • Accession Number:
      38002319