Paper-based electrodes as a tool for detecting ligninolytic enzymatic activities.

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
Read More Add to Saved list
  • Additional Information
    • Source:
      Publisher: Elsevier Country of Publication: Netherlands NLM ID: 100953583 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1878-562X (Electronic) Linking ISSN: 15675394 NLM ISO Abbreviation: Bioelectrochemistry Subsets: MEDLINE
    • Publication Information:
      Original Publication: [Amsterdam?] : Elsevier, 2000-
    • Subject Terms:
      Lignin*/chemistry ; Peroxidases*; Laccase ; Peroxidase ; Organic Chemicals/chemistry
    • Abstract:
      Lignin is the most important natural source of aromatic compounds. The valorisation of lignin into aromatics requires fractionation steps that can be catalysed by ligninolytic enzymes. However, one of the main limitations of biological lignin fractionation is the low efficiency of biocatalysts; it is therefore crucial to enhance or to identify new ligninolytic enzymes. Currently, the screening of ligninolytic activities on lignin polymers represents a technological bottenleck and hinders the characterization and the discovery of efficient ligninolytic biocatalysts. An efficient and fast method for the measurement of such enzymatic activities is therefore required. In this work, we present a new electrochemical tool based on lignin-coated paper electrodes for the detection and the characterization of ligninolytic activity. The suitability of this method is demonstrated using a catalase-peroxidase isolated from Thermobacillus xylanilyticus.
      Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
      (Copyright © 2023. Published by Elsevier B.V.)
    • Contributed Indexing:
      Keywords: Catalase-peroxidase; Depolymerization; Ligninolytic enzymes; Lignins; Paper-based electrodes
    • Accession Number:
      EC 1.10.3.2 (Laccase)
      9005-53-2 (Lignin)
      EC 1.11.1.7 (Peroxidase)
      EC 1.11.1.- (Peroxidases)
      0 (Organic Chemicals)
    • Publication Date:
      Date Created: 20231123 Date Completed: 20240117 Latest Revision: 20240117
    • Publication Date:
      20240117
    • Accession Number:
      10.1016/j.bioelechem.2023.108609
    • Accession Number:
      37995505