Genome analysis of haloalkaline isolates from the soda saline crater lake of Isabel Island; comparative genomics and potential metabolic analysis within the genus Halomonas.

Publisher: BioMed Central Country of Publication: England NLM ID: 100965258 Publication Model: Electronic Cited Medium: Internet ISSN: 1471-2164 (Electronic) Linking ISSN: 14712164 NLM ISO Abbreviation: BMC Genomics Subsets: MEDLINE

Original Publication: London : BioMed Central, [2000-

Halomonas*/genetics ; Halomonas*/metabolism; Lakes/microbiology ; Phylogeny ; Ecosystem ; Genomics ; Nitrogen/metabolism ; Phosphorus/metabolism ; RNA, Ribosomal, 16S/genetics ; DNA, Bacterial/genetics

Background: Isabel Island is a Mexican volcanic island primarily composed of basaltic stones. It features a maar known as Laguna Fragatas, which is classified as a meromictic thalassohaline lake. The constant deposition of guano in this maar results in increased levels of phosphorus, nitrogen, and carbon. The aim of this study was to utilize high-quality genomes from the genus Halomonas found in specialized databases as a reference for genome mining of moderately halophilic bacteria isolated from Laguna Fragatas. This research involved genomic comparisons employing phylogenetic, pangenomic, and metabolic-inference approaches.
Results: The Halomonas genus exhibited a large open pangenome, but several genes associated with salt metabolism and homeostatic regulation (ectABC and betABC), nitrogen intake through nitrate and nitrite transporters (nasA, and narGI), and phosphorus uptake (pstABCS) were shared among the Halomonas isolates.
Conclusions: The isolated bacteria demonstrate consistent adaptation to high salt concentrations, and their nitrogen and phosphorus uptake mechanisms are highly optimized. This optimization is expected in an extremophile environment characterized by minimal disturbances or abrupt seasonal variations. The primary significance of this study lies in the dearth of genomic information available for this saline and low-disturbance environment. This makes it important for ecosystem conservation and enabling an exploration of its biotechnological potential. Additionally, the study presents the first two draft genomes of H. janggokensis.
(© 2023. The Author(s).)

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Keywords: Genome mining; Halomonas; Halophilic lake; Metabolic capacities; Mexican lake

N762921K75 (Nitrogen)
27YLU75U4W (Phosphorus)
0 (RNA, Ribosomal, 16S)
0 (DNA, Bacterial)

Date Created: 20231121 Date Completed: 20231127 Latest Revision: 20231127

20231127

PMC10662389

10.1186/s12864-023-09800-9

37986038