Chemical profiling by UHPLC-Q-TOF-HRESI-MS/MS and antibacterial properties of Entada abyssinica (Fabaceae) constituents.

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  • Additional Information
    • Source:
      Publisher: Taylor & Francis Health Sciences Country of Publication: England NLM ID: 101167924 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1478-6427 (Electronic) Linking ISSN: 14786419 NLM ISO Abbreviation: Nat Prod Res Subsets: MEDLINE
    • Publication Information:
      Original Publication: Milton Park, UK : Taylor & Francis Health Sciences, c2003-
    • Subject Terms:
      Anti-Bacterial Agents*/pharmacology ; Anti-Bacterial Agents*/chemistry ; Fabaceae*/chemistry ; Microbial Sensitivity Tests* ; Tandem Mass Spectrometry*/methods; Chromatography, High Pressure Liquid/methods ; Molecular Structure ; Plants, Medicinal/chemistry ; Plant Extracts/chemistry ; Plant Extracts/pharmacology ; Sitosterols/chemistry ; Sitosterols/pharmacology ; Escherichia coli/drug effects ; Spectrometry, Mass, Electrospray Ionization ; Pseudomonas aeruginosa/drug effects
    • Abstract:
      A rapid untargeted UHPLC-Q-TOF-ESI-MS/MS-Based metabolomic profiling of the medicinal plant Entada abyssinica was performed. A total of 18 metabolites were detected, of which 10 could not be identified. Based on this result, an extensive chemical investigation of the CH 2 Cl 2 -MeOH (1:1) extract of this plant was carried out, leading to the isolation of a new ceramide, named entadamide ( 1 ), together with nine known compounds: monomethyl kolavate ( 2 ), 24-hydroxytormentic acid ( 3 ) chondrillasterol ( 4 ), 3- O - β -D glucopyranosylstigmasterol ( 5 ), 3- O - β -D glucopyranosylsitosterol ( 6 ), quercetin 3'-methylether ( 7 ), 2,3-dihydroxypropyl icosanoate ( 8 ), 2,3-dihydroxy-propyl 23-hydroxytricosanoate ( 9 ) and 2,3-dihydroxy-propyl 24-hydroxytetracosanoate ( 10 ). Their structures were elucidated by the analyses of their spectroscopic and spectrometric data (1D and 2D NMR, and HRESI-MS) in comparison with those reported in the literature. Furthermore, the crude extract and some isolated compounds were tested against non-ciprofloxacin resistant strains viz, Pseudomonas aeruginosa (ATCC 27853), Escherichia coli (ATCC 25922), Samonella thyphi (ATCC 19430) and Samonella enterica (NR4294). The tested samples demonstrated significant activity against all the tested bacteria (MIC values: 3.12-12.5 μg/mL).
    • Contributed Indexing:
      Keywords: Entada abyssinica; antibacterial activity; ceramide; metabolomics profiling
    • Accession Number:
      0 (Anti-Bacterial Agents)
      0 (Plant Extracts)
      0 (Sitosterols)
    • Publication Date:
      Date Created: 20231117 Date Completed: 20241110 Latest Revision: 20241110
    • Publication Date:
      20241114
    • Accession Number:
      10.1080/14786419.2023.2280171
    • Accession Number:
      37977828